1oj5

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[[Category: transcriptional regulation]]
[[Category: transcriptional regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:56:21 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:33:12 2007''

Revision as of 16:26, 12 November 2007

Image:1oj5.gif

1oj5, resolution 2.21Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF THE NCO-A1 PAS-B DOMAIN BOUND TO THE STAT6 TRANSACTIVATION DOMAIN LXXLL MOTIF

Overview

Signal transducer and activator of transcription 6 (STAT6) regulates, transcriptional activation in response to interleukin-4 (IL-4) by direct, interaction with coactivators. The CREB-binding protein (p300/CBP) and the, nuclear coactivator 1 (NCoA-1), a member of the p160/steroid receptor, coactivator family, bind independently to specific regions of the STAT6, transactivation domain and act as coactivators. The interaction between, STAT6 and NCoA-1 is mediated by an LXXLL motif in the transactivation, domain of STAT6. To define the mechanism of coactivator recognition, we, determined the crystal structure of the NCoA-1 PAS-B domain in complex, with the STAT6 LXXLL motif. The amphipathic, alpha-helical STAT6 LXXLL, motif binds mostly through specific hydrophobic interactions to NCoA-1. A, single amino acid of the NCoA-1 PAS-B domain establishes hydrophilic, interactions with the STAT6 peptide. STAT6 interacts only with the PAS-B, domain of NCoA-1 but not with the homologous regions of NCoA-2 and NCoA-3., The residues involved in binding the STAT6 peptide are strongly conserved, between the different NCoA family members. Therefore surface, complementarity between the hydrophobic faces of the STAT6 fragment and of, the NCoA-1 PAS-B domain almost exclusively defines the binding specificity, between the two proteins.

About this Structure

1OJ5 is a Protein complex structure of sequences from Mus musculus with IOD as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Structure of the NCoA-1/SRC-1 PAS-B domain bound to the LXXLL motif of the STAT6 transactivation domain., Razeto A, Ramakrishnan V, Litterst CM, Giller K, Griesinger C, Carlomagno T, Lakomek N, Heimburg T, Lodrini M, Pfitzner E, Becker S, J Mol Biol. 2004 Feb 13;336(2):319-29. PMID:14757047

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