2aud

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[[Image:2aud.gif|left|200px]]
[[Image:2aud.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2aud |SIZE=350|CAPTION= <scene name='initialview01'>2aud</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_2aud", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= hindIIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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|DOMAIN=
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{{STRUCTURE_2aud| PDB=2aud | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aud OCA], [http://www.ebi.ac.uk/pdbsum/2aud PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aud RCSB]</span>
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}}
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'''Unliganded HincII'''
'''Unliganded HincII'''
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[[Category: Horton, N C.]]
[[Category: Horton, N C.]]
[[Category: Little, E J.]]
[[Category: Little, E J.]]
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[[Category: blunt cutter]]
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[[Category: Blunt cutter]]
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[[Category: dna binding]]
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[[Category: Dna binding]]
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[[Category: restriction endonuclease]]
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[[Category: Restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:29:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:27 2008''
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Revision as of 16:29, 3 May 2008

Image:2aud.gif

Template:STRUCTURE 2aud

Unliganded HincII


Overview

The 2.1A crystal structure of the unliganded type II restriction endonuclease, HincII, is described. Although the asymmetric unit contains only a single monomer, crystal lattice contacts bring two monomers together to form a dimer very similar to that found in the DNA bound form. Comparison with the published DNA bound structure reveals that the DNA binding pocket is expanded in the unliganded structure. Comparison of the unliganded and DNA liganded structures reveals a simple rotation of subunits by 11 degrees each, or 22 degrees total, to a more closed structure around the bound DNA. Comparison of this conformational change to that observed in the other type II restriction endonucleases where DNA bound and unliganded forms have both been characterized, shows considerable variation in the types of conformational changes that can occur. The conformational changes in three can be described by a simple rotation of subunits, while in two others both rotation and translation of subunits relative to one another occurs. In addition, the endonucleases having subunits that undergo the greatest amount of rotation upon DNA binding are found to be those that distort the bound DNA the least, suggesting that DNA bending may be less facile in dimers possessing greater flexibility.

About this Structure

2AUD is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

DNA-induced conformational changes in type II restriction endonucleases: the structure of unliganded HincII., Little EJ, Horton NC, J Mol Biol. 2005 Aug 5;351(1):76-88. PMID:15993893 Page seeded by OCA on Sat May 3 19:29:18 2008

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