2avu

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[[Image:2avu.gif|left|200px]]
[[Image:2avu.gif|left|200px]]
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{{Structure
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|PDB= 2avu |SIZE=350|CAPTION= <scene name='initialview01'>2avu</scene>, resolution 3.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2avu", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|GENE= flhD, flbB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), flhC, flaI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_2avu| PDB=2avu | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2avu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avu OCA], [http://www.ebi.ac.uk/pdbsum/2avu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2avu RCSB]</span>
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'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription'''
'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription'''
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[[Category: Wang, S.]]
[[Category: Wang, S.]]
[[Category: Westbrook, E M.]]
[[Category: Westbrook, E M.]]
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[[Category: c4-type zinc finger]]
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[[Category: C4-type zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:31:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:58 2008''
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Revision as of 16:32, 3 May 2008

Template:STRUCTURE 2avu

Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription


Overview

The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC) regulates transcription from several flagellar and non-flagellar operons in bacteria. The crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution, revealing a hexameric FlhD4FlhC2 assembly. In the complex, each FlhC protomer binds an FlhD2 dimer; the conformation of the dimer in the complex differs significantly from its conformation in the absence of FlhC. FlhC has a novel tertiary fold that includes a heretofore unrecognized zinc-binding site in which the ion is ligated by four cysteine residues. Gel shift experiments show that binding of the FlhDC complex to a cognate promoter bends the DNA by approximately 111 degrees . The structure of the FlhDC complex is compatible with models in which a fragment of operator DNA, at least 48 base-pairs in length, wraps around the complex and bends significantly when binding.

About this Structure

2AVU is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:16337229 Page seeded by OCA on Sat May 3 19:31:59 2008

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