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| | ==Structure of the Krr1 and Faf1 complex from Saccharomyces cerevisiae== | | ==Structure of the Krr1 and Faf1 complex from Saccharomyces cerevisiae== |
| - | <StructureSection load='4qmf' size='340' side='right' caption='[[4qmf]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='4qmf' size='340' side='right'caption='[[4qmf]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qmf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qmf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QMF FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KRR1, YCL059C, YCL59C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), FAF1, YIL019W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmf OCA], [https://pdbe.org/4qmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qmf RCSB], [https://www.ebi.ac.uk/pdbsum/4qmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmf OCA], [http://pdbe.org/4qmf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qmf RCSB], [http://www.ebi.ac.uk/pdbsum/4qmf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KRR1_YEAST KRR1_YEAST]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.<ref>PMID:11996121</ref> <ref>PMID:11027267</ref> <ref>PMID:15094838</ref> <ref>PMID:15590835</ref> [[http://www.uniprot.org/uniprot/FAF1_YEAST FAF1_YEAST]] Required for pre-rRNA processing and 40S ribosomal subunit assembly. Seems to act in the processing of 35S rRNA at the A(0), A(1), and A(2) cleavage sites. | + | [https://www.uniprot.org/uniprot/KRR1_YEAST KRR1_YEAST] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.<ref>PMID:11996121</ref> <ref>PMID:11027267</ref> <ref>PMID:15094838</ref> <ref>PMID:15590835</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Ye, K]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Zheng, S]]
| + | [[Category: Ye K]] |
| - | [[Category: K-homology domain]] | + | [[Category: Zheng S]] |
| - | [[Category: Protein-protein complex]] | + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
KRR1_YEAST Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.[1] [2] [3] [4]
Publication Abstract from PubMed
Ribosome formation in Saccharomyces cerevisiae requires a large number of transiently associated assembly factors that coordinate processing and folding of pre-rRNA and binding of ribosomal proteins. Krr1 and Faf1 are two interacting proteins present in early 90 S precursor particles of the small ribosomal subunit. Here, we determined a co-crystal structure of the core domain of Krr1 bound to a 19-residue fragment of Faf1 at 2.8 A resolution. The structure reveals that Krr1 consists of two packed K homology (KH) domains, KH1 and KH2, and resembles archaeal Dim2-like proteins. We show that KH1 is a divergent KH domain that lacks the RNA-binding GXXG motif and is involved in binding another assembly factor, Kri1. KH2 contains a canonical RNA-binding surface and additionally associates with an alpha-helix of Faf1. Specific disruption of the Krr1-Faf1 interaction impaired early 18 S rRNA processing at sites A0, A1, and A2 and caused cell lethality, but it did not prevent incorporation of the two proteins into pre-ribosomes. The Krr1-Faf1 interaction likely maintains a critical conformation of 90 S pre-ribosomes required for pre-rRNA processing. Our results illustrate the versatility of KH domains in protein interaction and provide insight into the role of Krr1-Faf1 interaction in ribosome biogenesis.
Interaction between ribosome assembly factors Krr1 and Faf1 is essential for formation of small ribosomal subunit in yeast.,Zheng S, Lan P, Liu X, Ye K J Biol Chem. 2014 Aug 15;289(33):22692-703. doi: 10.1074/jbc.M114.584490. Epub, 2014 Jul 2. PMID:24990943[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gromadka R, Rytka J. The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae. Acta Biochim Pol. 2000;47(4):993-1005. PMID:11996121
- ↑ Sasaki T, Toh-E A, Kikuchi Y. Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus. Mol Cell Biol. 2000 Nov;20(21):7971-9. PMID:11027267
- ↑ Gromadka R, Karkusiewicz I, Rempola B, Rytka J. Functional and physical interactions of Krr1p, a Saccharomyces cerevisiae nucleolar protein. Acta Biochim Pol. 2004;51(1):173-87. PMID:15094838 doi:http://dx.doi.org/045101173
- ↑ Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ. The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell. 2004 Dec;3(6):1619-26. PMID:15590835 doi:http://dx.doi.org/10.1128/EC.3.6.1619-1626.2004
- ↑ Zheng S, Lan P, Liu X, Ye K. Interaction between ribosome assembly factors Krr1 and Faf1 is essential for formation of small ribosomal subunit in yeast. J Biol Chem. 2014 Aug 15;289(33):22692-703. doi: 10.1074/jbc.M114.584490. Epub, 2014 Jul 2. PMID:24990943 doi:http://dx.doi.org/10.1074/jbc.M114.584490
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