2ax2
From Proteopedia
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'''Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II''' | '''Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II''' | ||
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[[Category: Myles, D A.A.]] | [[Category: Myles, D A.A.]] | ||
[[Category: Silverman, D N.]] | [[Category: Silverman, D N.]] | ||
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Revision as of 16:34, 3 May 2008
Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II
Contents |
Overview
Human carbonic anhydrase II (HCA II) is a zinc metalloenzyme that catalyzes the reversible hydration and dehydration of carbon dioxide and bicarbonate, respectively. The rate-limiting step in catalysis is the intramolecular transfer of a proton between the zinc-bound solvent (H2O/OH-) and the proton-shuttling residue His64. This distance (approximately 7.5 A) is spanned by a well defined active-site solvent network stabilized by amino-acid side chains (Tyr7, Asn62, Asn67, Thr199 and Thr200). Despite the availability of high-resolution (approximately 1.0 A) X-ray crystal structures of HCA II, there is currently no definitive information available on the positions and orientations of the H atoms of the solvent network or active-site amino acids and their ionization states. In preparation for neutron diffraction studies to elucidate this hydrogen-bonding network, perdeuterated HCA II has been expressed, purified, crystallized and its X-ray structure determined to 1.5 A resolution. The refined structure is highly isomorphous with hydrogenated HCA II, especially with regard to the active-site architecture and solvent network. This work demonstrates the suitability of these crystals for neutron macromolecular crystallography.
Disease
Known disease associated with this structure: Osteopetrosis, autosomal recessive 3, with renal tubular acidosis OMIM:[611492]
About this Structure
2AX2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II., Budayova-Spano M, Fisher SZ, Dauvergne MT, Agbandje-McKenna M, Silverman DN, Myles DA, McKenna R, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jan 1;62(Pt 1):6-9., Epub 2005 Dec 16. PMID:16511248 Page seeded by OCA on Sat May 3 19:34:35 2008
