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2b4u

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[[Image:2b4u.gif|left|200px]]
[[Image:2b4u.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2b4u |SIZE=350|CAPTION= <scene name='initialview01'>2b4u</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2b4u", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= phyasr_C252S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=971 Selenomonas ruminantium])
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|DOMAIN=
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{{STRUCTURE_2b4u| PDB=2b4u | SCENE= }}
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|RELATEDENTRY=[[2b4o|2B4O]], [[2b4p|2B4P]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b4u OCA], [http://www.ebi.ac.uk/pdbsum/2b4u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b4u RCSB]</span>
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'''Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase'''
'''Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase'''
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[[Category: Mosimann, S C.]]
[[Category: Mosimann, S C.]]
[[Category: Selinger, L B.]]
[[Category: Selinger, L B.]]
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[[Category: ionic strength]]
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[[Category: Ionic strength]]
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[[Category: ptp-like]]
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[[Category: Ptp-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:51:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:32 2008''
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Revision as of 16:51, 3 May 2008

Image:2b4u.gif

Template:STRUCTURE 2b4u

Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase


Overview

PhyA from Selenomonas ruminantium (PhyAsr), is a bacterial protein tyrosine phosphatase (PTP)-like inositol polyphosphate phosphatase (IPPase) that is distantly related to known PTPs. PhyAsr has a second substrate binding site referred to as a standby site and the P-loop (HCX5R) has been observed in both open (inactive) and closed (active) conformations. Site-directed mutagenesis and kinetic and structural studies indicate PhyAsr follows a classical PTP mechanism of hydrolysis and has a broad specificity toward polyphosphorylated myo-inositol substrates, including phosphoinositides. Kinetic and molecular docking experiments demonstrate PhyAsr preferentially cleaves the 3-phosphate position of Ins P6 and will produce Ins(2)P via a highly ordered series of sequential dephosphorylations: D-Ins(1,2,4,5,6)P5, Ins(2,4,5,6)P4, D-Ins(2,4,5)P3, and D-Ins(2,4)P2. The data support a distributive enzyme mechanism and suggest the PhyAsr standby site is involved in the recruitment of substrate. Structural studies at physiological pH and high salt concentrations demonstrate the "closed" or active P-loop conformation can be induced in the absence of substrate. These results suggest PhyAsr should be reclassified as a D-3 myo-inositol hexakisphosphate phosphohydrolase and suggest the PhyAsr reaction mechanism is more similar to that of PTPs than previously suspected.

About this Structure

2B4U is a Single protein structure of sequence from Selenomonas ruminantium. Full crystallographic information is available from OCA.

Reference

Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase., Puhl AA, Gruninger RJ, Greiner R, Janzen TW, Mosimann SC, Selinger LB, Protein Sci. 2007 Jul;16(7):1368-78. Epub 2007 Jun 13. PMID:17567745 Page seeded by OCA on Sat May 3 19:51:27 2008

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