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5b03

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Revision as of 06:33, 31 May 2023

Structure of MoeN5-Sso7d fusion protein in complex with geranyl pyrophosphate

Structural highlights

5b03 is a 4 chain structure with sequence from Saccharolobus solfataricus P2 and Streptomyces viridosporus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A010_STRVD DN7D_SACS2 Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]^[1]

Publication Abstract from PubMed

The structure of MoeN5, a unique prenyltransferase involved in the biosynthesis of the antibiotic moenomycin, is reported. MoeN5 catalyzes the reaction of geranyl diphosphate (GPP) with the cis-farnesyl group in phosphoglycolipid 5 to form the (C25 ) moenocinyl-sidechain-containing lipid 7. GPP binds to an allylic site (S1) and aligns well with known S1 inhibitors. Alkyl glycosides, glycolipids, can bind to both S1 and a second site, S2. Long sidechains in S2 are "bent" and co-locate with the homoallylic substrate isopentenyl diphosphate in other prenyltransferases. These observations support a MoeN5 mechanism in which 5 binds to S2 with its C6-C11 group poised to attack C1 in GPP to form the moenocinyl sidechain, with the more distal regions of 5 aligning with the distal glucose in decyl maltoside. The results are of general interest because they provide the first structures of MoeN5 and a structural basis for its mechanism of action, results that will facilitate the design of new antibiotics.

Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5.,Zhang L, Chen CC, Ko TP, Huang JW, Zheng Y, Liu W, Wang I, Malwal SR, Feng X, Wang K, Huang CH, Hsu ST, Wang AH, Oldfield E, Guo RT Angew Chem Int Ed Engl. 2016 Apr 4;55(15):4716-20. doi: 10.1002/anie.201511388., Epub 2016 Mar 8. PMID:26954060^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kvaratskhelia M, Wardleworth BN, Bond CS, Fogg JM, Lilley DM, White MF. Holliday junction resolution is modulated by archaeal chromatin components in vitro. J Biol Chem. 2002 Jan 25;277(4):2992-6. Epub 2001 Nov 14. PMID:11709558 doi:http://dx.doi.org/10.1074/jbc.M109496200
↑ Zhang L, Chen CC, Ko TP, Huang JW, Zheng Y, Liu W, Wang I, Malwal SR, Feng X, Wang K, Huang CH, Hsu ST, Wang AH, Oldfield E, Guo RT. Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5. Angew Chem Int Ed Engl. 2016 Apr 4;55(15):4716-20. doi: 10.1002/anie.201511388., Epub 2016 Mar 8. PMID:26954060 doi:http://dx.doi.org/10.1002/anie.201511388

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5b03"

@@ Line 1: / Line 1: @@
 ==Structure of MoeN5-Sso7d fusion protein in complex with geranyl pyrophosphate==
-<StructureSection load='5b03' size='340' side='right' caption='[[5b03]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='5b03' size='340' side='right'caption='[[5b03]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5b03]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14672 Atcc 14672]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B03 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5b03]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B03 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPP:GERANYL+DIPHOSPHATE'>GPP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPP:GERANYL+DIPHOSPHATE'>GPP</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5b02|5b02]], [[5b00|5b00]], [[5b01|5b01]], [[5b0i|5b0i]], [[5b0j|5b0j]], [[5b0k|5b0k]], [[5b0l|5b0l]], [[5b0m|5b0m]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b03 OCA], [https://pdbe.org/5b03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b03 RCSB], [https://www.ebi.ac.uk/pdbsum/5b03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b03 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sso7d, sso7d-1, SSO10610 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35758 ATCC 14672])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b03 OCA], [http://pdbe.org/5b03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b03 RCSB], [http://www.ebi.ac.uk/pdbsum/5b03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b03 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A010_STRVD A0A010_STRVD] [https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 14672]]
+[[Category: Large Structures]]
-[[Category: Chen, C C]]
+[[Category: Saccharolobus solfataricus P2]]
-[[Category: Guo, R T]]
+[[Category: Streptomyces viridosporus]]
-[[Category: Ko, T P]]
+[[Category: Chen C-C]]
-[[Category: Oldfield, E O]]
+[[Category: Guo R-T]]
-[[Category: Zhang, L]]
+[[Category: Ko T-P]]
-[[Category: Alpha-helical fold]]
+[[Category: Oldfield EO]]
-[[Category: Dna binding protein]]
+[[Category: Zhang L]]
-[[Category: Fusion tag]]
-[[Category: Ligand complex]]
-[[Category: Prenyltransferase]]
-[[Category: Transferase]]

5b03

From Proteopedia

Revision as of 06:33, 31 May 2023

Structure of MoeN5-Sso7d fusion protein in complex with geranyl pyrophosphate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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