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4x3n

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Revision as of 21:10, 12 April 2023

Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum

Structural highlights

4x3n is a 3 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACTB_DICDI May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements.

Publication Abstract from PubMed

The crystal structure of the 34 kDa F-actin-bundling protein ABP34 from Dictyostelium discoideum was solved by Ca(2+)/S-SAD phasing and refined at 1.89 A resolution. ABP34 is a calcium-regulated actin-binding protein that cross-links actin filaments into bundles. Its in vitro F-actin-binding and F-actin-bundling activities were confirmed by a co-sedimentation assay and transmission electron microscopy. The co-localization of ABP34 with actin in cells was also verified. ABP34 adopts a two-domain structure with an EF-hand-containing N-domain and an actin-binding C-domain, but has no reported overall structural homologues. The EF-hand is occupied by a calcium ion with a pentagonal bipyramidal coordination as in the canonical EF-hand. The C-domain structure resembles a three-helical bundle and superposes well onto the rod-shaped helical structures of some cytoskeletal proteins. Residues 216-244 in the C-domain form part of the strongest actin-binding sites (193-254) and exhibit a conserved sequence with the actin-binding region of alpha-actinin and ABP120. Furthermore, the second helical region of the C-domain is kinked by a proline break, offering a convex surface towards the solvent area which is implicated in actin binding. The F-actin-binding model suggests that ABP34 binds to the side of the actin filament and residues 216-244 fit into a pocket between actin subdomains -1 and -2 through hydrophobic interactions. These studies provide insights into the calcium coordination in the EF-hand and F-actin-binding site in the C-domain of ABP34, which are associated through interdomain interactions.

Structure of the 34 kDa F-actin-bundling protein ABP34 from Dictyostelium discoideum.,Kim MK, Kim JH, Kim JS, Kang SO Acta Crystallogr D Biol Crystallogr. 2015 Sep;71(Pt 9):1835-49. doi:, 10.1107/S139900471501264X. Epub 2015 Aug 25. PMID:26327373^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kim MK, Kim JH, Kim JS, Kang SO. Structure of the 34 kDa F-actin-bundling protein ABP34 from Dictyostelium discoideum. Acta Crystallogr D Biol Crystallogr. 2015 Sep;71(Pt 9):1835-49. doi:, 10.1107/S139900471501264X. Epub 2015 Aug 25. PMID:26327373 doi:http://dx.doi.org/10.1107/S139900471501264X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4x3n"

@@ Line 1: / Line 1: @@
 ==Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum==
-<StructureSection load='4x3n' size='340' side='right' caption='[[4x3n]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
+<StructureSection load='4x3n' size='340' side='right'caption='[[4x3n]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4x3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X3N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4x3n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X3N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abpB, DDB_G0279081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x3n OCA], [https://pdbe.org/4x3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x3n RCSB], [https://www.ebi.ac.uk/pdbsum/4x3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x3n ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x3n OCA], [http://pdbe.org/4x3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x3n RCSB], [http://www.ebi.ac.uk/pdbsum/4x3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x3n ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACTB_DICDI ACTB_DICDI]] May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements.
+[https://www.uniprot.org/uniprot/ACTB_DICDI ACTB_DICDI] May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 11735]]
+[[Category: Dictyostelium discoideum]]
-[[Category: Kang, S O]]
+[[Category: Large Structures]]
-[[Category: Kim, J H]]
+[[Category: Kang S-O]]
-[[Category: Kim, J S]]
+[[Category: Kim J-H]]
-[[Category: Kim, M K]]
+[[Category: Kim J-S]]
-[[Category: Actin]]
+[[Category: Kim M-K]]
-[[Category: Bundling]]
-[[Category: Cytoskeleton]]
-[[Category: Protein binding]]

4x3n

From Proteopedia

Revision as of 21:10, 12 April 2023

Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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