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2bep
From Proteopedia
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[[Image:2bep.gif|left|200px]] | [[Image:2bep.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K''' | '''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K''' | ||
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[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: Sixma, T K.]] | [[Category: Sixma, T K.]] | ||
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Spine]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Structural proteomics in europe]] |
| - | [[Category: | + | [[Category: Ubiquitin,e2 conjugating enzyme,protein degradation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:17, 13 April 2008
CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K
Overview
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
About this Structure
2BEP is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079 Page seeded by OCA on Sun Apr 13 08:17:39 2008
Categories: Bos taurus | Single protein | Ubiquitin--protein ligase | Dijk, W J.Van. | Jentsch, S. | Knipscheer, P. | Korner, R. | Melchior, F. | Oberhofer, E. | Olsen, J Velgaard. | Pichler, A. | SPINE, Structural Proteomics in Europe. | Sixma, T K. | Ligase | Spine | Structural genomic | Structural proteomics in europe | Ubiquitin,e2 conjugating enzyme,protein degradation
