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| | ==High-resolution structure of two tandem B domains of staphylococcal protein A connected by the conserved linker== | | ==High-resolution structure of two tandem B domains of staphylococcal protein A connected by the conserved linker== |
| - | <StructureSection load='4npf' size='340' side='right' caption='[[4npf]], [[Resolution|resolution]] 1.49Å' scene=''> | + | <StructureSection load='4npf' size='340' side='right'caption='[[4npf]], [[Resolution|resolution]] 1.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4npf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NPF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4npf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NPF FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4npd|4npd]], [[4npe|4npe]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4npf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npf OCA], [https://pdbe.org/4npf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4npf RCSB], [https://www.ebi.ac.uk/pdbsum/4npf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4npf ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">protein A, spa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4npf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npf OCA], [http://pdbe.org/4npf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4npf RCSB], [http://www.ebi.ac.uk/pdbsum/4npf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4npf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deis, L N]] | + | [[Category: Large Structures]] |
| - | [[Category: IV, C W.Pemble]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Oas, T G]] | + | [[Category: Deis LN]] |
| - | [[Category: Richardson, D C]] | + | [[Category: Oas TG]] |
| - | [[Category: Richardson, J S]] | + | [[Category: Pemble IV CW]] |
| - | [[Category: Antibody]] | + | [[Category: Richardson DC]] |
| - | [[Category: Antibody binding]] | + | [[Category: Richardson JS]] |
| - | [[Category: Conformational heterogeneity]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Rapidly unfolding and folding]]
| + | |
| - | [[Category: Ruf]]
| + | |
| - | [[Category: Spa]]
| + | |
| - | [[Category: Three-helix bundle]]
| + | |
| - | [[Category: Tnfr1]]
| + | |
| - | [[Category: Von willebrand factor]]
| + | |
| Structural highlights
Function
SPA_STAAU
Publication Abstract from PubMed
The Staphylococcus aureus virulence factor staphylococcal protein A (SpA) is a major contributor to bacterial evasion of the host immune system, through high-affinity binding to host proteins such as antibodies. SpA includes five small three-helix-bundle domains (E-D-A-B-C) separated by conserved flexible linkers. Prior attempts to crystallize individual domains in the absence of a binding partner have apparently been unsuccessful. There have also been no previous structures of tandem domains. Here we report the high-resolution crystal structures of a single C domain, and of two B domains connected by the conserved linker. Both structures exhibit extensive multiscale conformational heterogeneity, which required novel modeling protocols. Comparison of domain structures shows that helix1 orientation is especially heterogeneous, coordinated with changes in side chain conformational networks and contacting protein interfaces. This represents the kind of structural plasticity that could enable SpA to bind multiple partners.
Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity.,Deis LN, Pemble CW 4th, Qi Y, Hagarman A, Richardson DC, Richardson JS, Oas TG Structure. 2014 Oct 7;22(10):1467-77. doi: 10.1016/j.str.2014.08.014. PMID:25295398[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deis LN, Pemble CW 4th, Qi Y, Hagarman A, Richardson DC, Richardson JS, Oas TG. Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity. Structure. 2014 Oct 7;22(10):1467-77. doi: 10.1016/j.str.2014.08.014. PMID:25295398 doi:http://dx.doi.org/10.1016/j.str.2014.08.014
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