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4yze

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Revision as of 07:32, 10 May 2023

Crystal structure of E.coli NemR reduced form

Structural highlights

4yze is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NEMR_ECOLI Represses the transcription of the nemRA operon by binding to the nemR box.^[1]

Publication Abstract from PubMed

Reactive chlorine species (RCS), such as hypochlorous acid (i.e., bleach), are antimicrobial oxidants produced by the innate immune system. Like many redox-regulated transcription factors, the Escherichia coli repressor NemR responds to RCS by using the reversible oxidation of highly conserved cysteines to alter its DNA-binding affinity. However, earlier work showed that RCS response in NemR does not depend on any commonly known oxidative cysteine modifications. We have now determined the crystal structure of NemR, showing that the regulatory cysteine, Cys106, is in close proximity to a highly conserved lysine (Lys175). We used crystallographic, biochemical, and mass spectrometric analyses to analyze the role of this lysine residue in RCS sensing. Based on our results, we hypothesize that RCS treatment of NemR results in the formation of a reversible Cys106-Lys175 sulfenamide bond. This is, to our knowledge, the first description of a protein whose function is regulated by a cysteine-lysine sulfenamide thiol switch, constituting a novel addition to the biological repertoire of functional redox switches.

Does the Transcription Factor NemR Use a Regulatory Sulfenamide Bond to Sense Bleach?,Gray MJ, Li Y, Leichert LI, Xu Z, Jakob U Antioxid Redox Signal. 2015 Sep 20;23(9):747-54. doi: 10.1089/ars.2015.6346. Epub, 2015 Jun 22. PMID:25867078^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Umezawa Y, Shimada T, Kori A, Yamada K, Ishihama A. The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase. J Bacteriol. 2008 Sep;190(17):5890-7. doi: 10.1128/JB.00459-08. Epub 2008 Jun 20. PMID:18567656 doi:http://dx.doi.org/10.1128/JB.00459-08
↑ Gray MJ, Li Y, Leichert LI, Xu Z, Jakob U. Does the Transcription Factor NemR Use a Regulatory Sulfenamide Bond to Sense Bleach? Antioxid Redox Signal. 2015 Sep 20;23(9):747-54. doi: 10.1089/ars.2015.6346. Epub, 2015 Jun 22. PMID:25867078 doi:http://dx.doi.org/10.1089/ars.2015.6346

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yze"

@@ Line 1: / Line 1: @@
 ==Crystal structure of E.coli NemR reduced form==
-<StructureSection load='4yze' size='340' side='right' caption='[[4yze]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='4yze' size='340' side='right'caption='[[4yze]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yze]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YZE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yze]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YZE FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nemR, ydhM, b1649, JW5874 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yze OCA], [https://pdbe.org/4yze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yze RCSB], [https://www.ebi.ac.uk/pdbsum/4yze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yze ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yze OCA], [http://pdbe.org/4yze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yze RCSB], [http://www.ebi.ac.uk/pdbsum/4yze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yze ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NEMR_ECOLI NEMR_ECOLI]] Represses the transcription of the nemRA operon by binding to the nemR box.<ref>PMID:18567656</ref>
+[https://www.uniprot.org/uniprot/NEMR_ECOLI NEMR_ECOLI] Represses the transcription of the nemRA operon by binding to the nemR box.<ref>PMID:18567656</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 17: @@
 </div>
 <div class="pdbe-citations 4yze" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Gray, M J]]
+[[Category: Large Structures]]
-[[Category: Jakob, U]]
+[[Category: Gray MJ]]
-[[Category: Li, Y]]
+[[Category: Jakob U]]
-[[Category: Xu, Z]]
+[[Category: Li Y]]
-[[Category: Cystein-lysine sulfenamide thiol]]
+[[Category: Xu Z]]
-[[Category: Transcription]]
-[[Category: Transcription factor]]

4yze

From Proteopedia

Revision as of 07:32, 10 May 2023

Crystal structure of E.coli NemR reduced form

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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