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2bkg
From Proteopedia
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[[Image:2bkg.gif|left|200px]] | [[Image:2bkg.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN''' | '''CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BKG is a [[Single protein]] structure | + | 2BKG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKG OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability., Binz HK, Kohl A, Pluckthun A, Grutter MG, Proteins. 2006 Nov 1;65(2):280-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16493627 16493627] | Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability., Binz HK, Kohl A, Pluckthun A, Grutter MG, Proteins. 2006 Nov 1;65(2):280-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16493627 16493627] | ||
| - | [[Category: ]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Binz, H K.]] | [[Category: Binz, H K.]] | ||
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[[Category: Kohl, A.]] | [[Category: Kohl, A.]] | ||
[[Category: Pluckthun, A.]] | [[Category: Pluckthun, A.]] | ||
| - | [[Category: | + | [[Category: Ankyrin repeat]] |
| - | [[Category: | + | [[Category: Consensus design]] |
| - | [[Category: | + | [[Category: Designed protein]] |
| - | [[Category: | + | [[Category: Protein stability]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:24:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:24, 3 May 2008
CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN
Overview
Consensus-designed ankyrin repeat (AR) proteins are thermodynamically very stable. The structural analysis of the designed AR protein E3_5 revealed that this stability is due to a regular fold with highly conserved structural motifs and H-bonding networks. However, the designed AR protein E3_19 exhibits a significantly lower stability than E3_5 (9.6 vs. 14.8 kcal/mol), despite 88% sequence identity. To investigate the structural correlations of this stability difference between E3_5 and E3_19, we determined the crystal structure of E3_19 at 1.9 A resolution. E3_19 as well has a regular AR domain fold with the characteristic H-bonding patterns. All structural features of the E3_5 and E3_19 molecules appear to be virtually identical (RMSD(Calpha) approximately 0.7 A). However, clear differences are observed in the surface charge distribution of the two AR proteins. E3_19 features clusters of charged residues and more exposed hydrophobic residues than E3_5. The atomic coordinates of E3_19 have been deposited in the Protein Data Bank. PDB ID: 2BKG.
About this Structure
2BKG is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability., Binz HK, Kohl A, Pluckthun A, Grutter MG, Proteins. 2006 Nov 1;65(2):280-4. PMID:16493627 Page seeded by OCA on Sat May 3 20:24:42 2008
