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2bod
From Proteopedia
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'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE''' | '''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE''' | ||
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[[Category: Roos, A.]] | [[Category: Roos, A.]] | ||
[[Category: Wilson, D B.]] | [[Category: Wilson, D B.]] | ||
| - | [[Category: | + | [[Category: Carbohydrate metabolism]] |
| - | [[Category: | + | [[Category: Cellulose degradation]] |
| - | [[Category: | + | [[Category: Endoglucanase]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase family 6]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Methyl cellobiosyl-4-thio-beta-cellobioside]] |
| - | [[Category: | + | [[Category: Polysaccharide degradation]] |
| - | [[Category: | + | [[Category: Thermobifida fusca]] |
| - | [[Category: | + | [[Category: Tim a/b fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:34:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:34, 3 May 2008
CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE
Overview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
About this Structure
2BOD is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.
Reference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060 Page seeded by OCA on Sat May 3 20:34:15 2008
Categories: Cellulase | Single protein | Thermobifida fusca | Bergfors, T. | Driguez, H. | Dultz, E. | Irwin, D C. | Jones, T A. | Larsson, A M. | Roos, A. | Wilson, D B. | Carbohydrate metabolism | Cellulose degradation | Endoglucanase | Glycosidase | Glycoside hydrolase family 6 | Hydrolase | Methyl cellobiosyl-4-thio-beta-cellobioside | Polysaccharide degradation | Tim a/b fold
