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6b6h

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Revision as of 10:14, 1 January 2020

The cryo-EM structure of a bacterial class I transcription activation complex

Structural highlights

6b6h is a 12 chain structure with sequence from Eco45, Eco57 and Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
NonStd Res:
Gene:	rpoA, Z4665, ECs4160 (ECO57), rpoB, ECS88_4448 (ECO45), rpoC, Z5561, ECs4911 (ECO57), rpoZ, ECS88_4064 (ECO45), rpoD, alt, b3067, JW3039 (ECOLI), crp, Z4718, ECs4208 (ECO57)
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CRP_ECO57] A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) (By similarity). [RPOC_ECO57] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [RPOA_ECO57] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [RPOB_ECO45] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [RPOZ_ECO45] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. [RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.

Publication Abstract from PubMed

In bacteria, the activation of gene transcription at many promoters is simple and only involves a single activator. The cyclic adenosine 3',5'-monophosphate receptor protein (CAP), a classic activator, is able to activate transcription independently through two different mechanisms. Understanding the class I mechanism requires an intact transcription activation complex (TAC) structure at a high resolution. Here we report a high-resolution cryo-electron microscopy structure of an intact Escherichia coli class I TAC containing a CAP dimer, a sigma(70)-RNA polymerase (RNAP) holoenzyme, a complete class I CAP-dependent promoter DNA, and a de novo synthesized RNA oligonucleotide. The structure shows how CAP wraps the upstream DNA and how the interactions recruit RNAP. Our study provides a structural basis for understanding how activators activate transcription through the class I recruitment mechanism.

Structural basis of bacterial transcription activation.,Liu B, Hong C, Huang RK, Yu Z, Steitz TA Science. 2017 Nov 17;358(6365):947-951. doi: 10.1126/science.aao1923. PMID:29146813^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu B, Hong C, Huang RK, Yu Z, Steitz TA. Structural basis of bacterial transcription activation. Science. 2017 Nov 17;358(6365):947-951. doi: 10.1126/science.aao1923. PMID:29146813 doi:http://dx.doi.org/10.1126/science.aao1923

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b6h"

@@ Line 1: / Line 1: @@
 ==The cryo-EM structure of a bacterial class I transcription activation complex==
-<StructureSection load='6b6h' size='340' side='right' caption='[[6b6h]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+<StructureSection load='6b6h' size='340' side='right'caption='[[6b6h]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6b6h]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco45 Eco45], [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6H FirstGlance]. <br>
@@ Line 21: / Line 21: @@
 </div>
 <div class="pdbe-citations 6b6h" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[RNA polymerase|RNA polymerase]]
+*[[Sigma factor|Sigma factor]]
 == References ==
 <references/>
@@ Line 29: / Line 33: @@
 [[Category: Eco57]]
 [[Category: Ecoli]]
+[[Category: Large Structures]]
 [[Category: Hong, C]]
 [[Category: Huang, R]]

6b6h

From Proteopedia

Revision as of 10:14, 1 January 2020

The cryo-EM structure of a bacterial class I transcription activation complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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