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2bz2
From Proteopedia
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[[Image:2bz2.gif|left|200px]] | [[Image:2bz2.gif|left|200px]] | ||
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'''SOLUTION STRUCTURE OF NELF E RRM''' | '''SOLUTION STRUCTURE OF NELF E RRM''' | ||
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[[Category: Schweimer, K.]] | [[Category: Schweimer, K.]] | ||
[[Category: Wohrl, B M.]] | [[Category: Wohrl, B M.]] | ||
| - | [[Category: | + | [[Category: Alternative splicing]] |
| - | [[Category: | + | [[Category: Nelf e]] |
| - | [[Category: | + | [[Category: Nuclear protein]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Repeat]] |
| - | [[Category: | + | [[Category: Repressor]] |
| - | [[Category: | + | [[Category: Rna recognition motif]] |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Transcription]] |
| - | [[Category: | + | [[Category: Transcription regulation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:59:52 2008'' | |
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Revision as of 17:59, 3 May 2008
SOLUTION STRUCTURE OF NELF E RRM
Overview
The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA polymerase II at the viral long terminal repeat. NELF is a heterotetrameric protein consisting of NELF A, B, C or the splice variant D, and E. In the present study, we determined the solution structure of the RRM (RNA-recognition motif) of the RNA-binding subunit NELF E and studied its interaction with the viral TAR RNA. Our results show that the separately expressed recombinant NELF E RRM has alpha-helical and beta-strand elements adopting a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA. Fluorescence equilibrium titrations with fluorescently labelled double- and single-stranded oligoribonucleotides representing the TAR RNA stem imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d) values in the low-micromolar range.
About this Structure
2BZ2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873 Page seeded by OCA on Sat May 3 20:59:52 2008
