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| | ==Crystal structure of Mycobacterium tuberculosis H37Rv orotate phosphoribosyltransferase in complex with inorganic phosphate== | | ==Crystal structure of Mycobacterium tuberculosis H37Rv orotate phosphoribosyltransferase in complex with inorganic phosphate== |
| - | <StructureSection load='5hkl' size='340' side='right' caption='[[5hkl]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='5hkl' size='340' side='right'caption='[[5hkl]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hkl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HKL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HKL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.899Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pyrE, umpA, Rv0382c, MTV036.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Orotate_phosphoribosyltransferase Orotate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.10 2.4.2.10] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hkl OCA], [https://pdbe.org/5hkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hkl RCSB], [https://www.ebi.ac.uk/pdbsum/5hkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hkl ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hkl OCA], [http://pdbe.org/5hkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hkl RCSB], [http://www.ebi.ac.uk/pdbsum/5hkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hkl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PYRE_MYCTU PYRE_MYCTU]] Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). | + | [https://www.uniprot.org/uniprot/PYRE_MYCTU PYRE_MYCTU] Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5hkl" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5hkl" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctu]] | + | [[Category: Large Structures]] |
| - | [[Category: Orotate phosphoribosyltransferase]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Bolognesi, G]] | + | [[Category: Bolognesi G]] |
| - | [[Category: Donini, S]] | + | [[Category: Donini S]] |
| - | [[Category: Rizzi, M]] | + | [[Category: Rizzi M]] |
| - | [[Category: De novo pyrimidine nucleotide synthesis]]
| + | |
| - | [[Category: Inorganic phosphate complex]]
| + | |
| - | [[Category: Oprt]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
PYRE_MYCTU Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
Publication Abstract from PubMed
The Mycobacterium tuberculosis orotate phosphoribosyltransferase (MtOPRT) catalyses the conversion of alpha-D-5-phosphoribosyl-1-pyrophosphate (PRPP) and orotate (OA) in pyrophosphate and orotidine 5'-monophosphate (OMP), in presence of Mg(2+). This enzyme is the only responsible for the synthesis of orotidine 5'-monophosphate, a key precursor in the de novo pyrimidine biosynthesis pathway, making MtOPRT an attractive drug target for the development of antitubercular agents. We report the crystal structures of MtOPRT in complex with PRPP (2.25 A resolution), inorganic phosphate (1.90 A resolution) and the exogenous compound Fe(III) dicitrate (2.40 A resolution). The overall structure of the mycobacterial enzyme is highly similar to those described for other OPRTases, with the "flexible loop" assuming a well define conformation and making specific contacts with the Fe(III)-dicitrate complex. The structures here reported add to the knowledge of a potential drug target for tuberculosis, and will provide a useful tool for the structure-based drug design of potent enzyme inhibitors.
Structural investigations on orotate phosphoribosyltransferase from Mycobacterium tuberculosis, a key enzyme of the de novo pyrimidine biosynthesis.,Donini S, Ferraris DM, Miggiano R, Massarotti A, Rizzi M Sci Rep. 2017 Apr 26;7(1):1180. doi: 10.1038/s41598-017-01057-z. PMID:28446777[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Donini S, Ferraris DM, Miggiano R, Massarotti A, Rizzi M. Structural investigations on orotate phosphoribosyltransferase from Mycobacterium tuberculosis, a key enzyme of the de novo pyrimidine biosynthesis. Sci Rep. 2017 Apr 26;7(1):1180. doi: 10.1038/s41598-017-01057-z. PMID:28446777 doi:http://dx.doi.org/10.1038/s41598-017-01057-z
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