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| | ==Gadolinium phased PBP2 (SSO6202) at 2.2 Ang== | | ==Gadolinium phased PBP2 (SSO6202) at 2.2 Ang== |
| - | <StructureSection load='5n35' size='340' side='right' caption='[[5n35]], [[Resolution|resolution]] 2.24Å' scene=''> | + | <StructureSection load='5n35' size='340' side='right'caption='[[5n35]], [[Resolution|resolution]] 2.24Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N35 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n35]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N35 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5n41|5n41]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSOP1_0579, SULA_1676, SULB_1677, SULC_1675 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n35 OCA], [https://pdbe.org/5n35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n35 RCSB], [https://www.ebi.ac.uk/pdbsum/5n35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n35 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n35 OCA], [http://pdbe.org/5n35 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n35 RCSB], [http://www.ebi.ac.uk/pdbsum/5n35 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n35 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0E3GTJ4_SACSO A0A0E3GTJ4_SACSO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 35091]] | + | [[Category: Large Structures]] |
| - | [[Category: Abrescia, N G.A]] | + | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: Albers, S V]] | + | [[Category: Abrescia NGA]] |
| - | [[Category: Beattie, T R]] | + | [[Category: Albers SV]] |
| - | [[Category: Bell, S D]] | + | [[Category: Beattie TR]] |
| - | [[Category: Gardner, A F]] | + | [[Category: Bell SD]] |
| - | [[Category: Gristwood, T]] | + | [[Category: Gardner AF]] |
| - | [[Category: Rojas, A L]] | + | [[Category: Gristwood T]] |
| - | [[Category: Roversi, P]] | + | [[Category: Rojas AL]] |
| - | [[Category: Schermerhorn, K]] | + | [[Category: Roversi P]] |
| - | [[Category: Trinidad, J C]] | + | [[Category: Schermerhorn K]] |
| - | [[Category: Yan, J]] | + | [[Category: Trinidad JC]] |
| - | [[Category: Archaeal dna polymerase holoenzyme]]
| + | [[Category: Yan J]] |
| - | [[Category: Polb1 binding protein 2]]
| + | |
| - | [[Category: Polymerase binding protein]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
A0A0E3GTJ4_SACSO
Publication Abstract from PubMed
Since their initial characterization over 30 years ago, it has been believed that the archaeal B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit B-family replicative polymerases of eukaryotes. Here we reveal that the highly studied PolB1 from Sulfolobus solfataricus exists as a heterotrimeric complex in cell extracts. Two small subunits, PBP1 and PBP2, associate with distinct surfaces of the larger catalytic subunit and influence the enzymatic properties of the DNA polymerase. Thus, multi-subunit replicative DNA polymerase holoenzymes are present in all three domains of life. We reveal the architecture of the assembly by a combination of cross-linking coupled with mass spectrometry, X-ray crystallography and single-particle electron microscopy. The small subunits stabilize the holoenzyme assembly and the acidic tail of one small subunit mitigates the ability of the enzyme to perform strand-displacement synthesis, with important implications for lagging strand DNA synthesis.
Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme.,Yan J, Beattie TR, Rojas AL, Schermerhorn K, Gristwood T, Trinidad JC, Albers SV, Roversi P, Gardner AF, Abrescia NGA, Bell SD Nat Commun. 2017 May 2;8:15075. doi: 10.1038/ncomms15075. PMID:28462924[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yan J, Beattie TR, Rojas AL, Schermerhorn K, Gristwood T, Trinidad JC, Albers SV, Roversi P, Gardner AF, Abrescia NGA, Bell SD. Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme. Nat Commun. 2017 May 2;8:15075. doi: 10.1038/ncomms15075. PMID:28462924 doi:http://dx.doi.org/10.1038/ncomms15075
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