1qox
From Proteopedia
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| - | [[Image:1qox.gif|left|200px]]<br /> | + | [[Image:1qox.gif|left|200px]]<br /><applet load="1qox" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1qox" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1qox, resolution 2.7Å" /> | caption="1qox, resolution 2.7Å" /> | ||
'''BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS'''<br /> | '''BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] | + | 1QOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] Known structural/functional Sites: <scene name='pdbsite=CAA:Active Site (Chain A)'>CAA</scene>, <scene name='pdbsite=CAB:Active Site (Chain B)'>CAB</scene>, <scene name='pdbsite=CAC:Active Site (Chain C)'>CAC</scene>, <scene name='pdbsite=CAD:Active Site (Chain D)'>CAD</scene>, <scene name='pdbsite=CAE:Active Site (Chain E)'>CAE</scene>, <scene name='pdbsite=CAF:Active Site (Chain F)'>CAF</scene>, <scene name='pdbsite=CAG:Active Site (Chain G)'>CAG</scene>, <scene name='pdbsite=CAH:Active Site (Chain H)'>CAH</scene>, <scene name='pdbsite=CAI:Active Site (Chain I)'>CAI</scene>, <scene name='pdbsite=CAJ:Active Site (Chain J)'>CAJ</scene>, <scene name='pdbsite=CAK:Active Site (Chain K)'>CAK</scene>, <scene name='pdbsite=CAL:Active Site (Chain L)'>CAL</scene>, <scene name='pdbsite=CAM:Active Site (Chain M)'>CAM</scene>, <scene name='pdbsite=CAN:Active Site (Chain N)'>CAN</scene>, <scene name='pdbsite=CAO:Active Site (Chain O)'>CAO</scene> and <scene name='pdbsite=CAP:Active Site (Chain P)'>CAP</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QOX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:58:32 2007'' |
Revision as of 15:48, 18 December 2007
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BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS
Overview
Family 1 of glycosyl hydrolases is a large and biologically important, group of enzymes. A new three-dimensional structure of this family, beta-glucosidase from Bacillus circulans sp. alkalophilus is reported, here. This is the first structure of beta-glucosidase from an alkaliphilic, organism. The model was determined by the molecular replacement method and, refined to a resolution of 2.7 A. The quaternary structure of B. circulans, sp. alkalophilus beta-glucosidase is an octamer and subunits of the, octamer show a similar (beta/alpha)(8) barrel fold to that previously, reported for other family 1 enzymes. The crystal structure suggested that, Cys169 in the active site is substituted. The Cys169 is located near the, putative acid/base catalyst Glu166 and it may contribute to the high pH, optimum of the enzyme. The crystal structure also revealed that the, asymmetric unit contains two octamers which have a clear binding, interaction with each other. The ability of the octamers to link with each, other suggested that beta-glucosidase from Bacillus circulans sp., alkalophilus is able to form long polymeric assemblies, at least in the, crystalline state.
About this Structure
1QOX is a Single protein structure of sequence from Bacillus circulans. Active as Beta-glucosidase, with EC number 3.2.1.21 Known structural/functional Sites: , , , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form long polymeric assemblies., Hakulinen N, Paavilainen S, Korpela T, Rouvinen J, J Struct Biol. 2000 Feb;129(1):69-79. PMID:10675298
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