5wk4
From Proteopedia
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<StructureSection load='5wk4' size='340' side='right' caption='[[5wk4]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='5wk4' size='340' side='right' caption='[[5wk4]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5wk4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Petma Petma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK4 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk4 OCA], [http://pdbe.org/5wk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk4 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk4 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk4 OCA], [http://pdbe.org/5wk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk4 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Variable lymphocyte receptors (VLRs), the leucine-rich repeat (LRR)-based antigen receptors of jawless fish, have great utility in a wide variety of biochemical and biological applications, similar to classical Ig-based antibodies. VLR-based reagents may be particularly useful when traditional antibodies are not available. An anti-idiotype lamprey VLR, VLR39, has previously been identified that recognizes the heavy-chain CDR3 of the B-cell receptor (BCR) of a leukemic clone from a patient with chronic lymphocytic leukemia (CLL). VLR39 was used successfully to track the re-emergence of this clone in the patient following chemotherapy. Here, the crystal structure of VLR39 is presented at 1.5 A resolution and compared with those of other protein-specific VLRs. VLR39 adopts a curved solenoid fold and exhibits substantial structural similarity to other protein-binding VLRs. VLR39 has a short LRRCT loop that protrudes outwards away from the concave face and is similar to those of its protein-specific VLR counterparts. Analysis of the VLR39-BCR interaction by size-exclusion chromatography and biolayer interferometry using the scFv version of the BCR confirms that VLR39 recognizes the BCR Fv region. Such VLR-based reagents may be useful for identifying and monitoring leukemia in CLL patients and in other clinical diagnostic assays. | ||
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| + | Crystal structure of an anti-idiotype variable lymphocyte receptor.,Collins BC, Nakahara H, Acharya S, Cooper MD, Herrin BR, Wilson IA Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):682-687. doi:, 10.1107/S2053230X1701620X. Epub 2017 Nov 14. PMID:29199989<ref>PMID:29199989</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5wk4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Petma]] | ||
[[Category: Collins, B C]] | [[Category: Collins, B C]] | ||
[[Category: Cooper, M D]] | [[Category: Cooper, M D]] | ||
Revision as of 06:44, 20 December 2017
Crystal structure of an anti-idiotype VLR
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