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| | ==Crystal structure of LuxF from Photobacterium leiognathi== | | ==Crystal structure of LuxF from Photobacterium leiognathi== |
| - | <StructureSection load='4j2p' size='340' side='right' caption='[[4j2p]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='4j2p' size='340' side='right'caption='[[4j2p]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j2p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25521 Atcc 25521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j2p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_leiognathi Photobacterium leiognathi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J2P FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nfp|1nfp]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2p OCA], [https://pdbe.org/4j2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j2p RCSB], [https://www.ebi.ac.uk/pdbsum/4j2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2p ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">luxF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553611 ATCC 25521])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2p OCA], [http://pdbe.org/4j2p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j2p RCSB], [http://www.ebi.ac.uk/pdbsum/4j2p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LUXF_PHOLE LUXF_PHOLE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 25521]] | + | [[Category: Large Structures]] |
| - | [[Category: Gruber, K]] | + | [[Category: Photobacterium leiognathi]] |
| - | [[Category: Macheroux, P]] | + | [[Category: Gruber K]] |
| - | [[Category: Winkler, A]] | + | [[Category: Macheroux P]] |
| - | [[Category: Incomplete beta-barrel]] | + | [[Category: Winkler A]] |
| - | [[Category: Luminescent protein]]
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| - | [[Category: Modified 7-stranded barrel]]
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| - | [[Category: Myr-fmn]]
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| - | [[Category: Myr-fmn binding]]
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| Structural highlights
Function
LUXF_PHOLE
Publication Abstract from PubMed
The lux-operon of bioluminescent bacteria contains the genes coding for the enzymes required for light emission. Some species of Photobacteria feature an additional gene, luxF, which shows similarity to luxA and luxB, the genes encoding the heterodimeric luciferase. Isolated dimeric LuxF binds four molecules of an unusually derivatized flavin, i.e., 6-(3'-(R)-myristyl)-FMN (myrFMN). In the present study we have heterologously expressed LuxF in Escherichia coli BL21 in order to advance our understanding of the protein's binding properties and its role in photobacterial bioluminescence. Structure determination by X-ray crystallography confirmed that apo-LuxF possesses four preorganized binding sites, which are further optimized by adjusting the orientation of amino acid side chains. To investigate the binding properties of recombinant LuxF we have isolated myrFMN from Photobacterium leiognathi S1. We found that LuxF binds myrFMN tightly with a dissociation constant of 80+/-20 nM demonstrating that the purified apo-form of LuxF is fully competent in myrFMN binding. In contrast to LuxF, binding of myrFMN to luciferase is much weaker (Kd=4.0+/-0.4 muM) enabling LuxF to prevent inhibition of the enzyme by scavenging myrFMN. Moreover, we have used apo-LuxF to demonstrate that myrFMN occurs in all Photobacteria tested, irrespective of the presence of luxF indicating that LuxF is not required for myrFMN biosynthesis.
Structural and biochemical properties of LuxF from Photobacterium leiognathi.,Bergner T, Tabib CR, Winkler A, Stipsits S, Kayer H, Lee J, Malthouse JP, Mayhew S, Muller F, Gruber K, Macheroux P Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1466-75. doi:, 10.1016/j.bbapap.2015.07.008. Epub 2015 Jul 21. PMID:26209460[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergner T, Tabib CR, Winkler A, Stipsits S, Kayer H, Lee J, Malthouse JP, Mayhew S, Muller F, Gruber K, Macheroux P. Structural and biochemical properties of LuxF from Photobacterium leiognathi. Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1466-75. doi:, 10.1016/j.bbapap.2015.07.008. Epub 2015 Jul 21. PMID:26209460 doi:http://dx.doi.org/10.1016/j.bbapap.2015.07.008
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