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| | ==PllA lectin, monosaccharide complex== | | ==PllA lectin, monosaccharide complex== |
| - | <StructureSection load='5odu' size='340' side='right' caption='[[5odu]], [[Resolution|resolution]] 1.56Å' scene=''> | + | <StructureSection load='5odu' size='340' side='right'caption='[[5odu]], [[Resolution|resolution]] 1.56Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5odu]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29999 Atcc 29999]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ODU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5odu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ODU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">plu2096 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29488 ATCC 29999])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5odu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odu OCA], [http://pdbe.org/5odu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5odu RCSB], [http://www.ebi.ac.uk/pdbsum/5odu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5odu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5odu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odu OCA], [https://pdbe.org/5odu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5odu RCSB], [https://www.ebi.ac.uk/pdbsum/5odu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5odu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PLLA_PHOLL PLLA_PHOLL] Lectin that specifically binds alpha-galactoside-terminating glycoconjugates. Shows high apparent binding to the alpha-Gal epitope (Gal-alpha-1,3-Gal-beta-1,4-GlcNAc terminating glycans) as well as to Gal-alpha-1,4-GlcNAc and Gal-alpha-1,3-GalNAc. Gal-alpha-1,3-GalNAc may be one natural ligand bound by PllA both in the nematode symbiont and in infected insects.<ref>PMID:28972138</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 29999]] | + | [[Category: Large Structures]] |
| - | [[Category: Koehnke, J]] | + | [[Category: Photorhabdus luminescens]] |
| - | [[Category: Sikandar, A]] | + | [[Category: Koehnke J]] |
| - | [[Category: Carbohydrate]] | + | [[Category: Sikandar A]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Plla]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
PLLA_PHOLL Lectin that specifically binds alpha-galactoside-terminating glycoconjugates. Shows high apparent binding to the alpha-Gal epitope (Gal-alpha-1,3-Gal-beta-1,4-GlcNAc terminating glycans) as well as to Gal-alpha-1,4-GlcNAc and Gal-alpha-1,3-GalNAc. Gal-alpha-1,3-GalNAc may be one natural ligand bound by PllA both in the nematode symbiont and in infected insects.[1]
Publication Abstract from PubMed
Lectins play important roles in infections by pathogenic bacteria, for example, in host colonization, persistence and biofilm formation. The Gram-negative entomopathogenic bacterium Photorhabdus luminescens symbiotically lives in insect-infecting Heterorhabditis nematodes and kills the insect host upon invasion by the nematode. The P. luminescens genome harbors the gene plu2096 coding for a novel lectin that we named PllA. We analyzed the binding properties of purified PllA with a glycan array and a binding assay in solution. Both assays revealed a strict specificity of PllA for alpha-galactoside-terminating glycoconjugates. The crystal structures of apo PllA and complexes with three different ligands revealed the molecular basis for the strict specificity of this lectin. Furthermore, we found that a 90 degree twist in subunit orientation leads to a peculiar quaternary structure compared with that of its ortholog LecA from Pseudomonas aeruginosa. We also investigated the utility of PllA as a probe for detecting alpha-galactosides. The alpha-Gal epitope is present on wild-type pig cells and the main reason for hyperacute organ rejection in pig to primate xenotransplantation. We noted that PllA specifically recognizes this epitope on the glycan array and demonstrated that PllA can be used as a fluorescent probe to detect this epitope on primary porcine cells in vitro. In summary, our biochemical and structural analyses of the P. luminescens lectin PllA have disclosed the structural basis for PllAs high specificity for alpha-galactoside-containing ligands, and we show that PllA can be used to visualize alpha-Gal epitope on porcine tissues.
Photorhabdus luminescens lectin A (PllA) - a new probe for detecting alpha-galactoside-terminating glycoconjugates.,Beshr G, Sikandar A, Jemiller EM, Klymiuk N, Hauck D, Wagner S, Wolf E, Koehnke J, Titz A J Biol Chem. 2017 Sep 28. pii: jbc.M117.812792. doi: 10.1074/jbc.M117.812792. PMID:28972138[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Beshr G, Sikandar A, Jemiller EM, Klymiuk N, Hauck D, Wagner S, Wolf E, Koehnke J, Titz A. Photorhabdus luminescens lectin A (PllA) - a new probe for detecting alpha-galactoside-terminating glycoconjugates. J Biol Chem. 2017 Sep 28. pii: jbc.M117.812792. doi: 10.1074/jbc.M117.812792. PMID:28972138 doi:http://dx.doi.org/10.1074/jbc.M117.812792
- ↑ Beshr G, Sikandar A, Jemiller EM, Klymiuk N, Hauck D, Wagner S, Wolf E, Koehnke J, Titz A. Photorhabdus luminescens lectin A (PllA) - a new probe for detecting alpha-galactoside-terminating glycoconjugates. J Biol Chem. 2017 Sep 28. pii: jbc.M117.812792. doi: 10.1074/jbc.M117.812792. PMID:28972138 doi:http://dx.doi.org/10.1074/jbc.M117.812792
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