2ccz

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[[Image:2ccz.gif|left|200px]]
[[Image:2ccz.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2ccz", creates the "Structure Box" on the page.
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{{STRUCTURE_2ccz| PDB=2ccz | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ccz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccz OCA], [http://www.ebi.ac.uk/pdbsum/2ccz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ccz RCSB]</span>
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'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''
'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''
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[[Category: Sun, Y J.]]
[[Category: Sun, Y J.]]
[[Category: Wu, H N.]]
[[Category: Wu, H N.]]
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[[Category: primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]]
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[[Category: Primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:50:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:19:46 2008''
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Revision as of 18:50, 3 May 2008

Image:2ccz.gif

Template:STRUCTURE 2ccz

CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA


Overview

PriB is a primosomal protein required for replication restart in Escherichia coli. PriB stimulates PriA helicase activity via interaction with single-stranded DNA (ssDNA), but the molecular details of this interaction remain unclear. Here, we report the crystal structure of PriB complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB shares structural similarity with the E.coli ssDNA-binding protein (EcoSSB). However, the structure of the PriB-dT15 complex reveals that PriB binds ssDNA differently. Results from filter-binding assays show that PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational analysis suggests that the loop L45 plays an important role in ssDNA binding. Based on the crystal structure and biochemical analyses, we propose a cooperative mechanism for the binding of PriB to ssDNA and a model for the assembly of the PriA-PriB-ssDNA complex. This report presents the first structure of a replication restart primosomal protein complexed with DNA, and a novel model that explains the interactions between a dimeric oligonucleotide-binding-fold protein and ssDNA.

About this Structure

2CCZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode., Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD, Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:16899446 Page seeded by OCA on Sat May 3 21:50:45 2008

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