2cfa
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2cfa.gif|left|200px]] | [[Image:2cfa.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2cfa", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_2cfa| PDB=2cfa | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX''' | '''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX''' | ||
| Line 25: | Line 22: | ||
[[Category: Paramecium bursaria chlorella virus 1]] | [[Category: Paramecium bursaria chlorella virus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Thymidylate synthase (FAD)]] | ||
[[Category: Bernauer, J.]] | [[Category: Bernauer, J.]] | ||
[[Category: Decottignies, P.]] | [[Category: Decottignies, P.]] | ||
| Line 36: | Line 32: | ||
[[Category: Tilbeurgh, H Van.]] | [[Category: Tilbeurgh, H Van.]] | ||
[[Category: Zhou, C Z.]] | [[Category: Zhou, C Z.]] | ||
| - | [[Category: | + | [[Category: Fdt]] |
| - | [[Category: | + | [[Category: Flavin dependent thymidylate synthase fad]] |
| - | [[Category: | + | [[Category: Flavoprotein]] |
| - | [[Category: | + | [[Category: Methyltransferase]] |
| - | [[Category: | + | [[Category: Nucleotide biosynthesis]] |
| - | [[Category: | + | [[Category: Paramecium bursaria chlorella virus-1]] |
| - | [[Category: | + | [[Category: Thyx]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | [[Category: | + | [[Category: Tscp]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:59:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:59, 3 May 2008
STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX
Overview
By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.
About this Structure
2CFA is a Protein complex structure of sequences from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:16707489 Page seeded by OCA on Sat May 3 21:59:40 2008
Categories: Paramecium bursaria chlorella virus 1 | Protein complex | Bernauer, J. | Decottignies, P. | Graille, M. | Graziani, S. | Liebl, U. | Marchand, C. | Myllykallio, H. | Skouloubris, S. | Tilbeurgh, H Van. | Zhou, C Z. | Fdt | Flavin dependent thymidylate synthase fad | Flavoprotein | Methyltransferase | Nucleotide biosynthesis | Paramecium bursaria chlorella virus-1 | Thyx | Transferase | Tscp
