1obn
From Proteopedia
(New page: 200px<br /> <applet load="1obn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1obn, resolution 1.30Å" /> '''ISOPENICILLIN N SYN...) |
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caption="1obn, resolution 1.30Å" /> | caption="1obn, resolution 1.30Å" /> | ||
'''ISOPENICILLIN N SYNTHASE AMINOADIPOYL-CYSTEINYL-AMINOBUTYRATE-FE-NO COMPLEX'''<br /> | '''ISOPENICILLIN N SYNTHASE AMINOADIPOYL-CYSTEINYL-AMINOBUTYRATE-FE-NO COMPLEX'''<br /> | ||
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| + | ==Overview== | ||
| + | Isopenicillin N synthase (IPNS) is a non-haem iron(II) oxidase which, catalyses the biosynthesis of isopenicillin N from the tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). Herein we report, crystallographic studies to investigate the reaction of IPNS with the, truncated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate (ACAb). It, has been reported previously that this analogue gives rise to three, beta-lactam products when incubated with IPNS: two methyl penams and a, cepham. Crystal structures of the IPNS-Fe(II)-ACAb and IPNS-Fe(II)-ACAb-NO, complexes have now been solved and are reported herein. These structures, and modelling studies based on them shed light on the diminished product, selectivity shown by IPNS in its reaction with ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12622704 (full description)]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OBN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4, ASV and NO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]]. | + | 1OBN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4, ASV and NO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]]. |
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| + | ==Reference== | ||
| + | Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate., Long AJ, Clifton IJ, Roach PL, Baldwin JE, Schofield CJ, Rutledge PJ, Biochem J. 2003 Jun 15;372(Pt 3):687-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12622704 12622704] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:11:37 2007'' |
Revision as of 09:06, 30 October 2007
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ISOPENICILLIN N SYNTHASE AMINOADIPOYL-CYSTEINYL-AMINOBUTYRATE-FE-NO COMPLEX
Overview
Isopenicillin N synthase (IPNS) is a non-haem iron(II) oxidase which, catalyses the biosynthesis of isopenicillin N from the tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). Herein we report, crystallographic studies to investigate the reaction of IPNS with the, truncated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate (ACAb). It, has been reported previously that this analogue gives rise to three, beta-lactam products when incubated with IPNS: two methyl penams and a, cepham. Crystal structures of the IPNS-Fe(II)-ACAb and IPNS-Fe(II)-ACAb-NO, complexes have now been solved and are reported herein. These structures, and modelling studies based on them shed light on the diminished product, selectivity shown by IPNS in its reaction with ... [(full description)]
About this Structure
1OBN is a [Single protein] structure of sequence from [Emericella nidulans] with FE2, SO4, ASV and NO as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate., Long AJ, Clifton IJ, Roach PL, Baldwin JE, Schofield CJ, Rutledge PJ, Biochem J. 2003 Jun 15;372(Pt 3):687-93. PMID:12622704
Page seeded by OCA on Tue Oct 30 11:11:37 2007
