1ruv
From Proteopedia
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| - | [[Image:1ruv.gif|left|200px]]<br /> | + | [[Image:1ruv.gif|left|200px]]<br /><applet load="1ruv" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ruv" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1ruv, resolution 1.25Å" /> | caption="1ruv, resolution 1.25Å" /> | ||
'''RIBONUCLEASE A-URIDINE VANADATE COMPLEX: HIGH RESOLUTION RESOLUTION X-RAY STRUCTURE (1.3 A)'''<br /> | '''RIBONUCLEASE A-URIDINE VANADATE COMPLEX: HIGH RESOLUTION RESOLUTION X-RAY STRUCTURE (1.3 A)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with UVC and TBU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | + | 1RUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with UVC and TBU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=ACT:The Site Is Defined As Residues Within 4 Angstroms Of U ...'>ACT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RUV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:59:42 2007'' |
Revision as of 15:49, 18 December 2007
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RIBONUCLEASE A-URIDINE VANADATE COMPLEX: HIGH RESOLUTION RESOLUTION X-RAY STRUCTURE (1.3 A)
Overview
The X-ray crystal structure of a uridine vanadate-ribonuclease A complex, has been determined at 1.3 A resolution. The resulting structure includes, all 124 amino-acid residues, a uridine vanadate, 131 water molecules, and, a single bound 2-methyl-2-propanol. Side chains of 11 surface residues, showing discrete disorder were modeled with multiple conformations. The, final crystallographic R factor is 0.197. Structures obtained from, high-level ab initio quantum calculations of model anionic oxyvanadate, compounds were used to probe the effects of starting structure on the, refinement process and final structure of the penta-coordinate phosphorane, analog, uridine vanadate. The least-squares refinement procedure gave rise, to the same final structure of the inhibitor despite significantly, different starting models. Comparison with the previously determined, complex of ribonuclease A with uridine vanadate obtained from a joint, X-ray/neutron analysis (6RSA) [Wlodawer, Miller & Sjolin (1983). Proc., Natl Acad. Sci. USA, 80, 3628-3631] reveals similarities in the overall, enzyme structure and the relative position of the key active-site, residues, Hisl2, His119 and Lys41, but significant differences in the V-O, bond distances and angles. The influence of ligand binding on the enzyme, structure is assessed by a comparison of the current X-ray structure with, the phosphate-free ribonuclease A structure (7RSA) [Wlodawer, Svensson, Sjolin & Gilliland (1988). Biochemistry, 27, 2705-2717]. Ligand binding, alters the solvent structure, distribution and number of residues with, multiple conformations, and temperature factors of the protein atoms. In, fact, the temperature factors of atoms of several residues that interact, with the ligand are reduced, but those of the atoms of several residues, remote from the active site exhibit marked increases.
About this Structure
1RUV is a Single protein structure of sequence from Bos taurus with UVC and TBU as ligands. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
X-ray structure of a ribonuclease A-uridine vanadate complex at 1.3 A resolution., Ladner JE, Wladkowski BD, Svensson LA, Sjolin L, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):290-301. PMID:15299932
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