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2cjk
From Proteopedia
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[[Image:2cjk.jpg|left|200px]] | [[Image:2cjk.jpg|left|200px]] | ||
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'''STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA''' | '''STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Perez-Canadillas, J M.]] | [[Category: Perez-Canadillas, J M.]] | ||
| - | [[Category: | + | [[Category: Cleavage]] |
| - | [[Category: | + | [[Category: Hrp1]] |
| - | [[Category: | + | [[Category: Mrna processing]] |
| - | [[Category: | + | [[Category: Nonsense-mediated mrna decay]] |
| - | [[Category: | + | [[Category: Nuclear protein]] |
| - | [[Category: | + | [[Category: Polyadenylation]] |
| - | [[Category: | + | [[Category: Rna processing]] |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Rna-binding protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:17:42 2008'' | |
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Revision as of 19:17, 3 May 2008
STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA
Overview
The recognition of specific signals encoded within the 3'-untranslated region of the newly transcribed mRNA triggers the assembly of a multiprotein machine that modifies its 3'-end. Hrp1 recognises one of such signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The molecular bases of this interaction are revealed here by the solution structure of a complex between Hrp1 and an oligonucleotide mimicking the PEE. Six consecutive bases (AUAUAU) are specifically recognised by two RNA-binding domains arranged in tandem. Both protein and RNA undergo significant conformational changes upon complex formation with a concomitant large surface burial of RNA bases. Key aspects of RNA specificity can be explained by the presence of intermolecular aromatic-aromatic contacts and hydrogen bonds. Altogether, the Hrp1-PEE structure represents one of the first steps towards understanding of the assembly of the cleavage and polyadenylation machinery at the atomic level.
About this Structure
2CJK is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1., Perez-Canadillas JM, EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:16794580 Page seeded by OCA on Sat May 3 22:17:42 2008
