2cjs

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[[Image:2cjs.gif|left|200px]]
[[Image:2cjs.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2cjs |SIZE=350|CAPTION= <scene name='initialview01'>2cjs</scene>, resolution 1.78&Aring;
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The line below this paragraph, containing "STRUCTURE_2cjs", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2cjs| PDB=2cjs | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjs OCA], [http://www.ebi.ac.uk/pdbsum/2cjs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cjs RCSB]</span>
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}}
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'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
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[[Category: Sudhof, T C.]]
[[Category: Sudhof, T C.]]
[[Category: Tomchick, D R.]]
[[Category: Tomchick, D R.]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: c2 domain]]
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[[Category: C2 domain]]
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[[Category: coiled coil]]
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[[Category: Coiled coil]]
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[[Category: exocytosis]]
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[[Category: Exocytosis]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: munc13]]
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[[Category: Munc13]]
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[[Category: neurotransmitter release]]
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[[Category: Neurotransmitter release]]
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[[Category: neurotransmitter transport]]
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[[Category: Neurotransmitter transport]]
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[[Category: phorbol-ester binding]]
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[[Category: Phorbol-ester binding]]
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[[Category: protein-protein interaction]]
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[[Category: Protein-protein interaction]]
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[[Category: rim]]
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[[Category: Rim]]
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[[Category: synapse]]
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[[Category: Synapse]]
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[[Category: synaptosome]]
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[[Category: Synaptosome]]
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[[Category: transport]]
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[[Category: Transport]]
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[[Category: zinc]]
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[[Category: Zinc]]
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[[Category: zinc finger]]
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[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:18:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:30 2008''
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Revision as of 19:18, 3 May 2008

Image:2cjs.gif

Template:STRUCTURE 2cjs

STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES


Overview

C(2) domains are well characterized as Ca(2+)/phospholipid-binding modules, but little is known about how they mediate protein-protein interactions. In neurons, a Munc13-1 C(2)A-domain/RIM zinc-finger domain (ZF) heterodimer couples synaptic vesicle priming to presynaptic plasticity. We now show that the Munc13-1 C(2)A domain homodimerizes, and that homodimerization competes with Munc13-1/RIM heterodimerization. X-ray diffraction studies guided by nuclear magnetic resonance (NMR) experiments reveal the crystal structures of the Munc13-1 C(2)A-domain homodimer and the Munc13-1 C(2)A-domain/RIM ZF heterodimer at 1.44 A and 1.78 A resolution, respectively. The C(2)A domain adopts a beta-sandwich structure with a four-stranded concave side that mediates homodimerization, leading to the formation of an eight-stranded beta-barrel. In contrast, heterodimerization involves the bottom tip of the C(2)A-domain beta-sandwich and a C-terminal alpha-helical extension, which wrap around the RIM ZF domain. Our results describe the structural basis for a Munc13-1 homodimer-Munc13-1/RIM heterodimer switch that may be crucial for vesicle priming and presynaptic plasticity, uncovering at the same time an unexpected versatility of C(2) domains as protein-protein interaction modules, and illustrating the power of combining NMR spectroscopy and X-ray crystallography to study protein complexes.

About this Structure

2CJS is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch., Lu J, Machius M, Dulubova I, Dai H, Sudhof TC, Tomchick DR, Rizo J, PLoS Biol. 2006 Jul;4(7):e192. PMID:16732694 Page seeded by OCA on Sat May 3 22:18:46 2008

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