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| | ==CRYSTAL STRUCTURE OF THE PTPN4 PDZ DOMAIN COMPLEXED WITH THE PDZ BINDING MOTIF OF THE MITOGEN ACTIVATED PROTEIN KINASE P38GAMMA.== | | ==CRYSTAL STRUCTURE OF THE PTPN4 PDZ DOMAIN COMPLEXED WITH THE PDZ BINDING MOTIF OF THE MITOGEN ACTIVATED PROTEIN KINASE P38GAMMA.== |
| - | <StructureSection load='5ez0' size='340' side='right' caption='[[5ez0]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='5ez0' size='340' side='right'caption='[[5ez0]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ez0]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EZ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ez0]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZ0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eyz|5eyz]], [[3nfk|3nfk]], [[3nfl|3nfl]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PTPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ez0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ez0 OCA], [https://pdbe.org/5ez0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ez0 RCSB], [https://www.ebi.ac.uk/pdbsum/5ez0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ez0 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ez0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ez0 OCA], [http://pdbe.org/5ez0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ez0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ez0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ez0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PTN4_HUMAN PTN4_HUMAN]] May act at junctions between the membrane and the cytoskeleton. | + | [https://www.uniprot.org/uniprot/PTN4_HUMAN PTN4_HUMAN] May act at junctions between the membrane and the cytoskeleton. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5ez0" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5ez0" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Protein-tyrosine-phosphatase]] | + | [[Category: Large Structures]] |
| - | [[Category: Caillet-Saguy, C]] | + | [[Category: Caillet-Saguy C]] |
| - | [[Category: Cordier, F]] | + | [[Category: Cordier F]] |
| - | [[Category: Delepierre, M]] | + | [[Category: Delepierre M]] |
| - | [[Category: Lafon, M]] | + | [[Category: Lafon M]] |
| - | [[Category: Maisonneuve, P]] | + | [[Category: Maisonneuve P]] |
| - | [[Category: Vaney, M C]] | + | [[Category: Vaney MC]] |
| - | [[Category: Wolff, N]] | + | [[Category: Wolff N]] |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Cell death]]
| + | |
| - | [[Category: Glioblastoma]]
| + | |
| - | [[Category: Mitogen activated protein kinase]]
| + | |
| - | [[Category: Multiprotein complex]]
| + | |
| - | [[Category: Non-receptor type 4]]
| + | |
| - | [[Category: P38gamma]]
| + | |
| - | [[Category: Pdz binding motif]]
| + | |
| - | [[Category: Pdz domain]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Ptpn4]]
| + | |
| - | [[Category: Rabies virus]]
| + | |
| Structural highlights
Function
PTN4_HUMAN May act at junctions between the membrane and the cytoskeleton.
Publication Abstract from PubMed
The human protein tyrosine phosphatase non-receptor type 4 (PTPN4) prevents cell death induction in neuroblastoma and glioblastoma cell lines in a PDZ-PDZ binding motifs (PBMs) dependent manner but the cellular partners of PTPN4 involved in cell protection are unknown. Here, we described the Mitogen-Activated protein kinase p38gamma as a cellular partner of PTPN4. The main contribution to the p38gamma:PTPN4 complex formation is the tight interaction between the C-terminus of p38gamma and the PDZ domain of PTPN4. We solved the crystal structure of the PDZ domain of PTPN4 bound to the p38gamma C-terminus. We identified the molecular basis of recognition of the C-terminal sequence of p38gamma that displays the highest affinity amongst all endogenous partners of PTPN4. We showed that the p38gamma C-terminus is also an efficient inducer of cell death after its intracellular delivery. In addition to recruiting the kinase, the binding of the C-terminal sequence of p38gamma to PTPN4 abolishes the catalytic auto-inhibition of PTPN4 and thus activates the phosphatase that can efficiently dephosphorylate the activation loop of p38gamma. We presume that the p38gamma-PTPN4 interaction promotes cellular signaling preventing cell death induction.
Molecular Basis of The Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-Activated Protein Kinase p38gamma.,Maisonneuve P, Caillet-Saguy C, Vaney MC, Edoo BZ, Sawyer K, Raynal B, Delepierre M, Lafon M, Cordier F, Wolff N J Biol Chem. 2016 May 31. pii: jbc.M115.707208. PMID:27246854[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Maisonneuve P, Caillet-Saguy C, Vaney MC, Edoo BZ, Sawyer K, Raynal B, Delepierre M, Lafon M, Cordier F, Wolff N. Molecular Basis of The Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-Activated Protein Kinase p38gamma. J Biol Chem. 2016 May 31. pii: jbc.M115.707208. PMID:27246854 doi:http://dx.doi.org/10.1074/jbc.M115.707208
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