5wdh

From Proteopedia

(Difference between revisions)

Current revision

Motor domain of human kinesin family member C1

Structural highlights

5wdh is a 1 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2rep. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.248Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KIFC1_HUMAN Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.^[1]

Publication Abstract from PubMed

Kinesin microtubule motor proteins play essential roles in division, including attaching chromosomes to spindles and crosslinking microtubules for spindle assembly. Human kinesin-14 KIFC1 is unique in that cancer cells with amplified centrosomes are dependent on the motor for viable division because of its ability to cluster centrosomes and form bipolar spindles, but it is not required for division in almost all normal cells. Screens for small molecule inhibitors of KIFC1 have yielded several candidates for further development, but obtaining structural data to determine their sites of binding has been difficult. Here we compare a previously unreported KIFC1 crystal structure with new structures of two closely related kinesin-14 proteins, Ncd and KIFC3, to determine the potential binding site of a known KIFC1 ATPase inhibitor, AZ82. We analyze the previously identified kinesin inhibitor binding sites and identify features of AZ82 that favor binding to one of the sites, the alpha4/alpha6 site. This selectivity can be explained by unique structural features of the KIFC1 alpha4/alpha6 binding site. These features may help improve the drug-like properties of AZ82 and other specific KIFC1 inhibitors.

Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein.,Park HW, Ma Z, Zhu H, Jiang S, Robinson RC, Endow SA Sci Rep. 2017 Nov 9;7(1):15121. doi: 10.1038/s41598-017-14754-6. PMID:29123223^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhu C, Zhao J, Bibikova M, Leverson JD, Bossy-Wetzel E, Fan JB, Abraham RT, Jiang W. Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference. Mol Biol Cell. 2005 Jul;16(7):3187-99. Epub 2005 Apr 20. PMID:15843429 doi:http://dx.doi.org/10.1091/mbc.E05-02-0167
↑ Park HW, Ma Z, Zhu H, Jiang S, Robinson RC, Endow SA. Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein. Sci Rep. 2017 Nov 9;7(1):15121. doi: 10.1038/s41598-017-14754-6. PMID:29123223 doi:http://dx.doi.org/10.1038/s41598-017-14754-6

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wdh"

@@ Line 1: / Line 1: @@
 ==Motor domain of human kinesin family member C1==
-<StructureSection load='5wdh' size='340' side='right' caption='[[5wdh]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='5wdh' size='340' side='right'caption='[[5wdh]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wdh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2rep 2rep]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WDH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WDH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wdh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2rep 2rep]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WDH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.248&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIFC1, HSET, KNSL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wdh OCA], [http://pdbe.org/5wdh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wdh RCSB], [http://www.ebi.ac.uk/pdbsum/5wdh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wdh ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wdh OCA], [https://pdbe.org/5wdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wdh RCSB], [https://www.ebi.ac.uk/pdbsum/5wdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wdh ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KIFC1_HUMAN KIFC1_HUMAN]] Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.<ref>PMID:15843429</ref>
+[https://www.uniprot.org/uniprot/KIFC1_HUMAN KIFC1_HUMAN] Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.<ref>PMID:15843429</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5wdh" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Kinesin 3D Structures|Kinesin 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Arrowsmith, C H]]
+[[Category: Large Structures]]
-[[Category: Brothers, G]]
+[[Category: Arrowsmith CH]]
-[[Category: Edwards, A M]]
+[[Category: Brothers G]]
-[[Category: He, H]]
+[[Category: Edwards AM]]
-[[Category: Landry, R]]
+[[Category: He H]]
-[[Category: Park, H]]
+[[Category: Landry R]]
-[[Category: Structural genomic]]
+[[Category: Park H]]
-[[Category: Shen, Y]]
+[[Category: Shen Y]]
-[[Category: Tempel, W]]
+[[Category: Tempel W]]
-[[Category: Wang, J]]
+[[Category: Wang J]]
-[[Category: Zhu, H]]
+[[Category: Zhu H]]
-[[Category: Adp]]
-[[Category: Kinesin]]
-[[Category: Motor domain]]
-[[Category: Motor protein]]
-[[Category: Sgc]]

5wdh

From Proteopedia

Current revision

Motor domain of human kinesin family member C1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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