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| | ==Crystal structure of the Schizogenesis pombe kinetochore Mis12C subcomplex== | | ==Crystal structure of the Schizogenesis pombe kinetochore Mis12C subcomplex== |
| - | <StructureSection load='5wwl' size='340' side='right' caption='[[5wwl]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5wwl' size='340' side='right'caption='[[5wwl]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wwl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wwl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WWL FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mis12, SPBC409.04c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), nnf1, SPAC29E6.04, SPAC30.08 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwl OCA], [http://pdbe.org/5wwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wwl RCSB], [http://www.ebi.ac.uk/pdbsum/5wwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwl OCA], [https://pdbe.org/5wwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wwl RCSB], [https://www.ebi.ac.uk/pdbsum/5wwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MIS12_SCHPO MIS12_SCHPO]] Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Required for correct segregation of chromosomes and for maintaining the inner centromere structure.<ref>PMID:12773390</ref> [[http://www.uniprot.org/uniprot/NNF1_SCHPO NNF1_SCHPO]] Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. | + | [https://www.uniprot.org/uniprot/MIS12_SCHPO MIS12_SCHPO] Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Required for correct segregation of chromosomes and for maintaining the inner centromere structure.<ref>PMID:12773390</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Kinetochores are superprotein complexes that orchestrate chromosome segregation via a dynamic interaction with spindle microtubules. A physical connection between CENP-C and the Mis12-Ndc80-Knl1 (KMN) protein network is an important pathway that is used to assemble kinetochores on CENP-A nucleosomes. Multiple outer kinetochore components are phosphorylated by Aurora B kinase to activate the spindle assembly checkpoint (SAC) and to ensure accurate chromosome segregation. However, it is unknown whether Aurora B can phosphorylate inner kinetochore components to facilitate proper mitotic chromosome segregation. Here, we reported the structure of the fission yeast Schizosaccharomyces pombe Mis12-Nnf1 complex and showed that N-terminal residues 26-50 in Cnp3 (the CENP-C homolog of S. pombe) are responsible for interacting with the Mis12 complex. Interestingly, Thr28 of Cnp3 is a substrate of Ark1 (the Aurora B homolog of S. pombe), and phosphorylation impairs the interaction between the Cnp3 and Mis12 complex. The expression of a phosphorylation-mimicking Cnp3 mutant results in defective chromosome segregation due to improper kinetochore assembly. These results establish a previously uncharacterized regulatory mechanism involved in CENP-C-Mis12-facilitated kinetochore attachment error correction to ensure accurate chromosome segregation during mitosis.
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| - | Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.,Zhou X, Zheng F, Wang C, Wu M, Zhang X, Wang Q, Yao X, Fu C, Zhang X, Zang J Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10667-E10676. doi:, 10.1073/pnas.1710506114. Epub 2017 Nov 27. PMID:29180432<ref>PMID:29180432</ref>
| + | ==See Also== |
| - | | + | *[[Centromere protein 3D structure|Centromere protein 3D structure]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5wwl" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Wang, C]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Wu, M]] | + | [[Category: Wang C]] |
| - | [[Category: Zang, J]] | + | [[Category: Wu M]] |
| - | [[Category: Zhang, X]] | + | [[Category: Zang J]] |
| - | [[Category: Zhou, X]] | + | [[Category: Zhang X]] |
| - | [[Category: Cell cycle]]
| + | [[Category: Zhou X]] |
| - | [[Category: Kinetochore mis12c]]
| + | |
