2cl2

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[[Image:2cl2.jpg|left|200px]]
[[Image:2cl2.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2cl2 |SIZE=350|CAPTION= <scene name='initialview01'>2cl2</scene>, resolution 1.35&Aring;
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The line below this paragraph, containing "STRUCTURE_2cl2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,3(4)-beta-glucanase Endo-1,3(4)-beta-glucanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.6 3.2.1.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2cl2| PDB=2cl2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cl2 OCA], [http://www.ebi.ac.uk/pdbsum/2cl2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cl2 RCSB]</span>
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}}
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'''ENDO-1,3(4)-BETA-GLUCANASE FROM PHANEROCHAETE CHRYSOSPORIUM, SOLVED USING NATIVE SULFUR SAD, EXHIBITING INTACT HEPTASACCHARIDE GLYCOSYLATION'''
'''ENDO-1,3(4)-BETA-GLUCANASE FROM PHANEROCHAETE CHRYSOSPORIUM, SOLVED USING NATIVE SULFUR SAD, EXHIBITING INTACT HEPTASACCHARIDE GLYCOSYLATION'''
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==Reference==
==Reference==
X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium., Vasur J, Kawai R, Larsson AM, Igarashi K, Sandgren M, Samejima M, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1422-9. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057348 17057348]
X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium., Vasur J, Kawai R, Larsson AM, Igarashi K, Sandgren M, Samejima M, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1422-9. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057348 17057348]
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[[Category: Endo-1,3(4)-beta-glucanase]]
 
[[Category: Phanerochaete chrysosporium]]
[[Category: Phanerochaete chrysosporium]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Stahlberg, J.]]
[[Category: Stahlberg, J.]]
[[Category: Vasur, J.]]
[[Category: Vasur, J.]]
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[[Category: basidiomycete]]
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[[Category: Basidiomycete]]
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[[Category: beta sandwich]]
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[[Category: Beta sandwich]]
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[[Category: beta-1,3/1,6-glucan]]
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[[Category: Beta-1,3/1,6-glucan]]
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[[Category: beta-glucanase]]
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[[Category: Beta-glucanase]]
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[[Category: extracellular]]
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[[Category: Extracellular]]
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[[Category: family 16]]
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[[Category: Family 16]]
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[[Category: gh16]]
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[[Category: Gh16]]
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[[Category: gh7]]
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[[Category: Gh7]]
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[[Category: glycosyl hydrolase]]
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[[Category: Glycosyl hydrolase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: lam16a,pichea pastori]]
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[[Category: Lam16a,pichea pastori]]
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[[Category: laminarin]]
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[[Category: Laminarin]]
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[[Category: laminarinase]]
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[[Category: Laminarinase]]
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[[Category: white rot fungus]]
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[[Category: White rot fungus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:23:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:59 2008''
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Revision as of 19:23, 3 May 2008

Image:2cl2.jpg

Template:STRUCTURE 2cl2

ENDO-1,3(4)-BETA-GLUCANASE FROM PHANEROCHAETE CHRYSOSPORIUM, SOLVED USING NATIVE SULFUR SAD, EXHIBITING INTACT HEPTASACCHARIDE GLYCOSYLATION


Overview

Laminarinase Lam16A from Phanerochaete chrysosporium was recombinantly expressed in Pichia pastoris, crystallized and the structure was solved at 1.34 A resolution using native sulfur SAD X-ray crystallography. It is the first structure of a non-specific 1,3(4)-beta-D-glucanase from glycoside hydrolase family 16 (GH16). P. chrysosporium is a wood-degrading basidiomycete fungus and Lam16A is the predominant extracellular protein expressed when laminarin is used as the sole carbon source. The protein folds into a curved beta-sandwich homologous to those of other known GH16 enzyme structures (especially kappa-carrageenase from Pseudoalteromonas carrageenovora and beta-agarase from Zobelia galactanivorans). A notable likeness is also evident with the related glycoside hydrolase family 7 (GH7) enzymes. A mammalian lectin, p58/ERGIC, as well as polysaccharide lyase (PL7) enzymes also showed significant similarity to Lam16A. The enzyme has two potential N-glycosylation sites. One such site, at Asn43, displayed a branched heptasaccharide sufficiently stabilized to be interpreted from the X-ray diffraction data. The other N-glycosylation motif was found close to the catalytic centre and is evidently not glycosylated.

About this Structure

2CL2 is a Single protein structure of sequence from Phanerochaete chrysosporium. Full crystallographic information is available from OCA.

Reference

X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium., Vasur J, Kawai R, Larsson AM, Igarashi K, Sandgren M, Samejima M, Stahlberg J, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1422-9. Epub 2006, Oct 18. PMID:17057348 Page seeded by OCA on Sat May 3 22:23:47 2008

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