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1ezg

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Revision as of 10:24, 18 November 2020

CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR

Structural highlights

1ezg is a 2 chain structure with sequence from Tenmo. The December 2009 RCSB PDB Molecule of the Month feature on Antifreeze Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2009_12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1eww
Gene:	CDNA FROM FAT BODY LIBRARY (TENMO)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ANPY1_TENMO] Contributes to protect body fluid from freezing at subzero temperatures. Lowers the freezing point of the hemolymph by about 2.5 degrees at a concentration of 1 mg/ml. Binds to nascent ice crystals and prevents further growth.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Insect antifreeze proteins (AFP) are much more effective than fish AFPs at depressing solution freezing points by ice-growth inhibition. AFP from the beetle Tenebrio molitor is a small protein (8.4 kDa) composed of tandem 12-residue repeats (TCTxSxxCxxAx). Here we report its 1.4-A resolution crystal structure, showing that this repetitive sequence translates into an exceptionally regular beta-helix. Not only are the 12-amino-acid loops almost identical in the backbone, but also the conserved side chains are positioned in essentially identical orientations, making this AFP perhaps the most regular protein structure yet observed. The protein has almost no hydrophobic core but is stabilized by numerous disulphide and hydrogen bonds. On the conserved side of the protein, threonine-cysteine-threonine motifs are arrayed to form a flat beta-sheet, the putative ice-binding surface. The threonine side chains have exactly the same rotameric conformation and the spacing between OH groups is a near-perfect match to the ice lattice. Together with tightly bound co-planar external water, three ranks of oxygen atoms form a two-dimensional array, mimicking an ice section.

Mimicry of ice structure by surface hydroxyls and water of a beta-helix antifreeze protein.,Liou YC, Tocilj A, Davies PL, Jia Z Nature. 2000 Jul 20;406(6793):322-4. PMID:10917536^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liou YC, Thibault P, Walker VK, Davies PL, Graham LA. A complex family of highly heterogeneous and internally repetitive hyperactive antifreeze proteins from the beetle Tenebrio molitor. Biochemistry. 1999 Aug 31;38(35):11415-24. PMID:10471292 doi:http://dx.doi.org/10.1021/bi990613s
↑ Liou YC, Daley ME, Graham LA, Kay CM, Walker VK, Sykes BD, Davies PL. Folding and structural characterization of highly disulfide-bonded beetle antifreeze protein produced in bacteria. Protein Expr Purif. 2000 Jun;19(1):148-57. PMID:10833402 doi:http://dx.doi.org/10.1006/prep.2000.1219
↑ Graham LA, Liou YC, Walker VK, Davies PL. Hyperactive antifreeze protein from beetles. Nature. 1997 Aug 21;388(6644):727-8. PMID:9285581 doi:http://dx.doi.org/10.1038/41908
↑ Liou YC, Tocilj A, Davies PL, Jia Z. Mimicry of ice structure by surface hydroxyls and water of a beta-helix antifreeze protein. Nature. 2000 Jul 20;406(6793):322-4. PMID:10917536 doi:10.1038/35018604

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ezg"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR==
-<StructureSection load='1ezg' size='340' side='right' caption='[[1ezg]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+<StructureSection load='1ezg' size='340' side='right'caption='[[1ezg]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ezg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tenmo Tenmo]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EZG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ezg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tenmo Tenmo]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1EZG FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eww|1eww]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CDNA FROM FAT BODY LIBRARY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7067 TENMO])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ezg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezg OCA], [http://pdbe.org/1ezg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ezg RCSB], [http://www.ebi.ac.uk/pdbsum/1ezg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezg ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ezg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezg OCA], [http://pdbe.org/1ezg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ezg RCSB], [http://www.ebi.ac.uk/pdbsum/1ezg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 1ezg" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
 == References ==
 <references/>
@@ Line 34: / Line 37: @@
 </StructureSection>
 [[Category: Antifreeze Proteins]]
+[[Category: Large Structures]]
 [[Category: RCSB PDB Molecule of the Month]]
 [[Category: Tenmo]]

1ezg

From Proteopedia

Revision as of 10:24, 18 November 2020

CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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