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1f08

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Revision as of 14:26, 16 October 2019

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

Structural highlights

1f08 is a 2 chain structure with sequence from Bovine papillomavirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori.

Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus.,Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L Mol Cell. 2000 Jul;6(1):149-58. PMID:10949036^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L. Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus. Mol Cell. 2000 Jul;6(1):149-58. PMID:10949036

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f08"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS==
-<StructureSection load='1f08' size='340' side='right' caption='[[1f08]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='1f08' size='340' side='right'caption='[[1f08]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1f08]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F08 FirstGlance]. <br>
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Bovine papillomavirus]]
+[[Category: Large Structures]]
 [[Category: Chen, G]]
 [[Category: Enemark, E J]]

1f08

From Proteopedia

Revision as of 14:26, 16 October 2019

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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