1sky

From Proteopedia

Revision as of 15:50, 18 December 2007

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

Overview

BACKGROUND: F1-ATPase, an oligomeric assembly with subunit stoichiometry, alpha 3 beta 3 gamma delta epsilon, is the catalytic component of the ATP, synthase complex, which plays a central role in energy transduction in, bacteria, chloroplasts and mitochondria. The crystal structure of bovine, mitochondrial F1-ATPase displays a marked asymmetry in the conformation, and nucleotide content of the catalytic beta subunits. The alpha 3 beta 3, subcomplex of F1-ATPase has been assembled from subunits of the moderately, thermophilic Bacillus PS3 made in Escherichia coli, and the subcomplex is, active but does not show the catalytic cooperativity of intact F1-ATPase., The structure of this subcomplex should provide new information on the, conformational variability of F1-ATPase and may provide insights into the, unusual catalytic mechanism employed by this enzyme. RESULTS: The crystal, structure of the nucleotide-free bacterial alpha 3 beta 3 subcomplex of, F1-ATPase, determined at 3.2 A resolution, shows that the oligomer has, exact threefold symmetry. The bacterial beta subunits adopt a conformation, essentially identical to that of the nucleotide-free beta subunit in, mitochondrial F1-ATPase; the alpha subunits have similar conformations in, both structures. CONCLUSIONS: The structures of the bacterial F1-ATPase, alpha and beta subunits are very similar to their counterparts in the, mitochondrial enzyme, suggesting a common catalytic mechanism. The study, presented here allows an analysis of the different conformations adopted, by the alpha and beta subunits and may ultimately further our, understanding of this mechanism.

About this Structure

1SKY is a Protein complex structure of sequences from Bacillus sp. ps3 with SO4 as ligand. Active as Transferred entry: 3.6.3.14, with EC number 3.6.1.34 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer., Shirakihara Y, Leslie AG, Abrahams JP, Walker JE, Ueda T, Sekimoto Y, Kambara M, Saika K, Kagawa Y, Yoshida M, Structure. 1997 Jun 15;5(6):825-36. PMID:9261073

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