2cmt
From Proteopedia
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'''THE STRUCTURE OF REDUCED CYCLOPHILIN A FROM S. MANSONI''' | '''THE STRUCTURE OF REDUCED CYCLOPHILIN A FROM S. MANSONI''' | ||
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[[Category: Gourlay, L J.]] | [[Category: Gourlay, L J.]] | ||
[[Category: Miele, A E.]] | [[Category: Miele, A E.]] | ||
| - | [[Category: | + | [[Category: Beta-barrel]] |
| - | [[Category: | + | [[Category: Cyclophilin]] |
| - | [[Category: | + | [[Category: Cyclosporin]] |
| - | [[Category: | + | [[Category: Isomerase]] |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Rotamase]] |
| - | [[Category: | + | [[Category: Rotamase activity]] |
| - | [[Category: | + | [[Category: Schistosoma]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:31:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:31, 3 May 2008
THE STRUCTURE OF REDUCED CYCLOPHILIN A FROM S. MANSONI
Overview
Treatment of schistosomiasis, a widespread human parasitic disease caused by the helminth parasites of the genus Schistosoma, relies mainly on one chemotherapeutic agent, praziquantel, although several other compounds exert anti-parasitic effects. One such compound is the immunosuppressant cyclosporin A, which has been shown to significantly diminish worm burden in mice infected with Schistosoma mansoni. Given the well established interaction between cyclosporin A and the cyclophilin superfamily of peptidylprolyl cis-trans isomerases, we solved the structure of cyclophilin A from S. mansoni (SmCypA) by x-ray crystallography in the reduced and oxidized states at 1.5 and 1.8 A of resolution, respectively. Oxidized SmCypA contains a disulfide bridge between two C-terminal cysteines (Cys-122 and Cys-126). This is the first example of a cyclophilin containing this disulfide bridge. Parallel functional studies suggest a mechanism for regulation of SmCypA activity via oxidation of its thiol groups; in fact, whereas oxidized SmCypA is inactive, reduced SmCypA is an efficient isomerase active at nanomolar levels with a k(cat)/K(m) of 1.1 x 10(7) M(-1) s(-1), and it is inhibited by cyclosporin A (IC(50) of 14 +/- 4 nM). The lack of conservation of this cysteine couple within the CypA superfamily, their close proximity to the active site, and the importance of thiol groups for peptidyl-prolyl cis-trans isomerase activity render this structural feature a challenge for the development of alternative and more effective anti-schistosomiasis inhibitors and may in addition imply an alternative function of SmCypA in the schistosome.
About this Structure
2CMT is a Single protein structure of sequence from Schistosoma mansoni. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of two redox states of cyclophilin A from Schistosoma mansoni. Evidence for redox regulation of peptidyl-prolyl cis-trans isomerase activity., Gourlay LJ, Angelucci F, Baiocco P, Boumis G, Brunori M, Bellelli A, Miele AE, J Biol Chem. 2007 Aug 24;282(34):24851-7. Epub 2007 Jun 25. PMID:17591771 Page seeded by OCA on Sat May 3 22:31:47 2008
