6fan
From Proteopedia
(Difference between revisions)
m (Protected "6fan" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of putative CooT from Carboxydothermus hydrogenoformans== | |
| + | <StructureSection load='6fan' size='340' side='right' caption='[[6fan]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6fan]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Carhz Carhz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAN FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CHY_0178 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246194 CARHZ])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fan OCA], [http://pdbe.org/6fan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fan RCSB], [http://www.ebi.ac.uk/pdbsum/6fan PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fan ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Carboxydothermus hydrogenoformans is a model microorganism for the study of [NiFe]-CODH, a key enzyme of carbon cycle in anaerobic microorganisms. The enzyme possesses a unique active site (C-cluster), constituted of a distorted [NiFe3S4] cubane linked to a mononuclear Fe(II) center. Both the biogenesis of the C-cluster and the activation of CODH by nickel insertion remain unclear. Among the three accessory proteins thought to play a role in this latter step (CooC, CooJ, and CooT), CooT is identified as a nickel chaperone involved in CODH maturation in Rhodospirillum rubrum. Here, we structurally and biophysically characterized a putative CooT protein present in C. hydrogenoformans (pChCooT). Despite the low sequence homologies between CooT from R. rubrum (RrCooT) and pChCooT (19% sequence identity), the two proteins share several similarities, such as their overall structure and a solvent-exposed Ni(II)-binding site at the dimer interface. Moreover, the X-ray structure of pChCooT reveals the proximity between the histidine 55, a potential nickel-coordinating residue, and the cysteine 2, a highly conserved key residue in Ni(II)-binding. | ||
| - | + | Biophysical and structural characterization of the putative nickel chaperone CooT from Carboxydothermus hydrogenoformans.,Alfano M, Perard J, Miras R, Catty P, Cavazza C J Biol Inorg Chem. 2018 Jun 7. pii: 10.1007/s00775-018-1576-2. doi:, 10.1007/s00775-018-1576-2. PMID:29882029<ref>PMID:29882029</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 6fan" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Carhz]] | ||
[[Category: Alfano, M]] | [[Category: Alfano, M]] | ||
| + | [[Category: Cavazza, C]] | ||
| + | [[Category: Carbon monoxide dehydrogenase]] | ||
| + | [[Category: Maturation pathway]] | ||
| + | [[Category: Metal binding protein]] | ||
| + | [[Category: Nickel chaperone]] | ||
Revision as of 20:29, 2 December 2018
Crystal structure of putative CooT from Carboxydothermus hydrogenoformans
| |||||||||||
