6fbu
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli (E2Q) in complex with AP-site containing DNA substrate== | |
| - | + | <StructureSection load='6fbu' size='340' side='right' caption='[[6fbu]], [[Resolution|resolution]] 2.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[6fbu]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBU FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |
| - | [[ | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PED:PENTANE-3,4-DIOL-5-PHOSPHATE'>PED</scene></td></tr> |
| - | [[ | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ea0|2ea0]], [[2opf|2opf]], [[2oq4|2oq4]]</td></tr> |
| - | [[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fbu OCA], [http://pdbe.org/6fbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fbu RCSB], [http://www.ebi.ac.uk/pdbsum/6fbu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fbu ProSAT]</span></td></tr> |
| - | [[ | + | </table> |
| - | [[ | + | == Function == |
| + | [[http://www.uniprot.org/uniprot/END8_ECOLI END8_ECOLI]] Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Acts on DNA bubble and 3'-fork structures, suggesting a role in replication-associated DNA repair. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.<ref>PMID:20031487</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Golan, G]] | [[Category: Golan, G]] | ||
| + | [[Category: Grollman, A P]] | ||
[[Category: Lansky, S]] | [[Category: Lansky, S]] | ||
| + | [[Category: Pomyalov, S]] | ||
| + | [[Category: Shoham, G]] | ||
| + | [[Category: Zharkov, D O]] | ||
| + | [[Category: Covalent intermediate]] | ||
| + | [[Category: Dna repair enzyme]] | ||
| + | [[Category: Endonuclease-viii]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Nei]] | ||
Current revision
Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli (E2Q) in complex with AP-site containing DNA substrate
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