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6em9

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Revision as of 08:49, 3 October 2018

S.aureus ClpC resting state, asymmetric map

Structural highlights

6em9 is a 10 chain structure with sequence from Staab. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	clpC, SAB0475 (STAAB)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CLPC_STAAB] Required for growth at high temperatures, probably by acting as a chaperone during heat shock and targeting heat-denatured proteins for degradation by ClpP.

Publication Abstract from PubMed

Ring-forming AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore. This activity is potentially harmful requiring mechanisms for tight repression and substrate-specific activation. The AAA+ chaperone ClpC with the peptidase ClpP forms a bacterial protease essential to virulence and stress resistance. The adaptor MecA activates ClpC by targeting substrates and stimulating ClpC ATPase activity. We show how ClpC is repressed in its ground state by determining ClpC cryo-EM structures with and without MecA. ClpC forms large two-helical assemblies that associate via head-to-head contacts between coiled-coil middle domains (MDs). MecA converts this resting state to an active planar ring structure by binding to MD interaction sites. Loss of ClpC repression in MD mutants causes constitutive activation and severe cellular toxicity. These findings unravel an unexpected regulatory concept executed by coiled-coil MDs to tightly control AAA+ chaperone activity.

Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control.,Carroni M, Franke KB, Maurer M, Jager J, Hantke I, Gloge F, Linder D, Gremer S, Turgay K, Bukau B, Mogk A Elife. 2017 Nov 22;6. doi: 10.7554/eLife.30120. PMID:29165246^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Carroni M, Franke KB, Maurer M, Jager J, Hantke I, Gloge F, Linder D, Gremer S, Turgay K, Bukau B, Mogk A. Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control. Elife. 2017 Nov 22;6. doi: 10.7554/eLife.30120. PMID:29165246 doi:http://dx.doi.org/10.7554/eLife.30120

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6em9"

@@ Line 1: / Line 1: @@
-{{Large structure}}
 ==S.aureus ClpC resting state, asymmetric map==
 <StructureSection load='6em9' size='340' side='right' caption='[[6em9]], [[Resolution|resolution]] 8.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6em9]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EM9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6em9]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EM9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6em9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6em9 OCA], [http://pdbe.org/6em9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6em9 RCSB], [http://www.ebi.ac.uk/pdbsum/6em9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6em9 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clpC, SAB0475 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6em9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6em9 OCA], [http://pdbe.org/6em9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6em9 RCSB], [http://www.ebi.ac.uk/pdbsum/6em9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6em9 ProSAT]</span></td></tr>
 </table>
-{{Large structure}}
 == Function ==
 [[http://www.uniprot.org/uniprot/CLPC_STAAB CLPC_STAAB]] Required for growth at high temperatures, probably by acting as a chaperone during heat shock and targeting heat-denatured proteins for degradation by ClpP.
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Staab]]
 [[Category: Bukau, B]]
 [[Category: Carroni, M]]

6em9

From Proteopedia

Revision as of 08:49, 3 October 2018

S.aureus ClpC resting state, asymmetric map

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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