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| | ==Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)== | | ==Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)== |
| - | <StructureSection load='5oqn' size='340' side='right' caption='[[5oqn]], [[Resolution|resolution]] 3.15Å' scene=''> | + | <StructureSection load='5oqn' size='340' side='right'caption='[[5oqn]], [[Resolution|resolution]] 3.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5oqn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5oqn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OQN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YCG1, YCS5, YDR325W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), BRN1, YBL097W, YBL0830 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqn OCA], [http://pdbe.org/5oqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqn RCSB], [http://www.ebi.ac.uk/pdbsum/5oqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqn OCA], [https://pdbe.org/5oqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oqn RCSB], [https://www.ebi.ac.uk/pdbsum/5oqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CND3_YEAST CND3_YEAST]] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase. [[http://www.uniprot.org/uniprot/CND2_YEAST CND2_YEAST]] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.<ref>PMID:10749930</ref> <ref>PMID:10749931</ref> | + | [https://www.uniprot.org/uniprot/CND3_YEAST CND3_YEAST] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5oqn" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5oqn" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Condensin|Condensin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Haering, C H]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Hassler, M]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Kschonsak, M]] | + | [[Category: Haering CH]] |
| - | [[Category: Cell cycle]] | + | [[Category: Hassler M]] |
| - | [[Category: Dna-binding]] | + | [[Category: Kschonsak M]] |
| - | [[Category: Heat repeat]]
| + | |
| - | [[Category: Kleisin]]
| + | |
| - | [[Category: Smc complex]]
| + | |
| Structural highlights
Function
CND3_YEAST Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.
Publication Abstract from PubMed
Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1Cnd3 HEAT-repeat and Brn1Cnd2 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the kleisin loop around DNA is essential for the association of condensin complexes with chromosomes and their DNA-stimulated ATPase activity. Our data suggest a sophisticated molecular basis for anchoring condensin complexes to chromosomes that enables the formation of large-sized chromatin loops.
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.,Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH. Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes. Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770 doi:http://dx.doi.org/10.1016/j.cell.2017.09.008
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