2cvq

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[[Image:2cvq.gif|left|200px]]
[[Image:2cvq.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2cvq |SIZE=350|CAPTION= <scene name='initialview01'>2cvq</scene>, resolution 2.08&Aring;
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The line below this paragraph, containing "STRUCTURE_2cvq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2cvq| PDB=2cvq | SCENE= }}
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|RELATEDENTRY=[[1bmd|1BMD]], [[1y7t|1Y7T]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvq OCA], [http://www.ebi.ac.uk/pdbsum/2cvq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cvq RCSB]</span>
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}}
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'''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''
'''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''
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[[Category: Nishiyama, M.]]
[[Category: Nishiyama, M.]]
[[Category: Tomita, T.]]
[[Category: Tomita, T.]]
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[[Category: nad-dependent-mdh nadp+ complex]]
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[[Category: Nad-dependent-mdh nadp+ complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:09:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:27:00 2008''
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Revision as of 20:09, 3 May 2008

Template:STRUCTURE 2cvq

Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH


Overview

For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.

About this Structure

2CVQ is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)., Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M, Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. PMID:16009341 Page seeded by OCA on Sat May 3 23:09:52 2008

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