2cxc
From Proteopedia
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'''Crystal structure of archaeal transcription termination factor NusA''' | '''Crystal structure of archaeal transcription termination factor NusA''' | ||
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[[Category: Shirouzu, M.]] | [[Category: Shirouzu, M.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| - | [[Category: | + | [[Category: Archaeal nusa]] |
| - | [[Category: | + | [[Category: Kh domain]] |
| - | [[Category: | + | [[Category: National project on protein structural and functional analyse]] |
| - | [[Category: | + | [[Category: Nppsfa]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rna binding protein]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Transcription termination]] |
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Revision as of 20:16, 3 May 2008
Crystal structure of archaeal transcription termination factor NusA
Overview
The transcription factor NusA functions in transcriptional regulation involving termination in bacteria. A NusA homolog consisting of only the two KH domains is widely conserved in archaea, but its function remains unknown. We have found that Aeropyrum pernix NusA strongly binds to a certain CU-rich sequence near a termination signal. Our crystal structure of A. pernix NusA revealed that its spatial arrangement is quite similar to that of the KH domains of bacterial NusA. Thus, we consider archaeal NusA to have retained some functions of bacterial NusA, including the ssRNA-binding ability. Remarkable structural differences between archaeal and bacterial NusA exist at the interface with RNAP, in connection with the different NusA-binding sites around the termination signals. Transcriptional termination in archaea could differ from all of the known bacterial and eukaryal mechanisms, in terms of the combination of a bacterial factor and a eukaryal-type RNAP.
About this Structure
2CXC is a Single protein structure of sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.
Reference
Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA., Shibata R, Bessho Y, Shinkai A, Nishimoto M, Fusatomi E, Terada T, Shirouzu M, Yokoyama S, Biochem Biophys Res Commun. 2007 Mar 30;355(1):122-8. Epub 2007 Jan 31. PMID:17288993 Page seeded by OCA on Sat May 3 23:16:54 2008
Categories: Aeropyrum pernix | Single protein | Bessho, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shibata, R. | Shirouzu, M. | Yokoyama, S. | Archaeal nusa | Kh domain | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rna binding protein | Rsgi | Structural genomic | Transcription termination
