2d03

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[[Image:2d03.gif|left|200px]]
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{{Structure
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|PDB= 2d03 |SIZE=350|CAPTION= <scene name='initialview01'>2d03</scene>, resolution 1.97&Aring;
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The line below this paragraph, containing "STRUCTURE_2d03", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
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{{STRUCTURE_2d03| PDB=2d03 | SCENE= }}
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|RELATEDENTRY=[[1t2q|1T2Q]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d03 OCA], [http://www.ebi.ac.uk/pdbsum/2d03 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d03 RCSB]</span>
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'''Crystal structure of the G91S mutant of the NNA7 Fab'''
'''Crystal structure of the G91S mutant of the NNA7 Fab'''
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[[Category: Wedekind, J E.]]
[[Category: Wedekind, J E.]]
[[Category: Xie, K.]]
[[Category: Xie, K.]]
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[[Category: antibody]]
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[[Category: Antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:27:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:38 2008''
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Revision as of 20:27, 3 May 2008

Image:2d03.gif

Template:STRUCTURE 2d03

Crystal structure of the G91S mutant of the NNA7 Fab


Overview

The NNA7 Fab antibody fragment recognizes the human N-type blood-group antigen comprised of the N-terminal glycopeptide of glycophorin A (GPA). A mutant form of this Fab fragment, NNA7-G91S, exhibits markedly reduced antigen binding. To provide insight into how these Fab fragments recognize this glycopeptide antigen, the crystal structures of NNA7 and NNA7-G91S were solved and refined to 1.83 and 1.97 A resolution, respectively. Both molecules are composed of the same heavy (H) chain Fd fragment, but each contains a slightly different light (L) chain owing to the G91S substitution. Specifically, the G91S mutation pushes the backbone of the neighboring H chain away from complementarity-determining region 3 (CDR3) of the L-chain variable region, allowing an additional glycerol cryoprotectant molecule to enter the antigen-combining site near the putative location of O-linked glycosylation. Each Fab fragment also possesses a well defined 2-(N-morpholino)ethanesulfonic acid (MES) molecule trapped in its antigen-combining site, as well as a crystallographic symmetry-related molecule comprising an amino-acid sequence that is virtually identical to the N-terminus of GPA. The MES molecule interacts with the H-chain CDR in a manner reminiscent of antibody-carbohydrate complexes. These results suggest a model for recognition of the glycopeptide antigen that accounts for the deleterious effect of the G91S substitution.

About this Structure

2D03 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of the NNA7 Fab and proposal for the mode of human blood-group recognition., Xie K, Song SC, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1386-94. Epub 2005, Sep 28. PMID:16204891 Page seeded by OCA on Sat May 3 23:27:07 2008

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