2d3d
From Proteopedia
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'''crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1''' | '''crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1''' | ||
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[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
[[Category: Zhao, X S.]] | [[Category: Zhao, X S.]] | ||
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| - | [[Category: | + | [[Category: Sam domain]] |
| - | [[Category: | + | [[Category: Sre hairpin binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:37:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:37, 3 May 2008
crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1
Overview
The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1 has a high affinity towards RNA hairpins containing a CUGGC pentaloop. We present the 1.6 Angstroms X-ray crystal structure of the Vts1 SAM domain in its unliganded state, and the NMR solution structure of this domain in its RNA-bound state. Both structures reveal a canonical five helix SAM domain flanked by additional secondary structural elements at the N and C termini. The two structures are essentially identical, implying that no major structural rearrangements occur upon RNA binding. Amide chemical shift changes map the RNA-binding site to a shallow, basic patch at the junction of helix alpha5 and the loop connecting helices alpha1 and alpha2.
About this Structure
2D3D is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The NMR and X-ray structures of the Saccharomyces cerevisiae Vts1 SAM domain define a surface for the recognition of RNA hairpins., Aviv T, Amborski AN, Zhao XS, Kwan JJ, Johnson PE, Sicheri F, Donaldson LW, J Mol Biol. 2006 Feb 17;356(2):274-9. Epub 2005 Dec 7. PMID:16375924 Page seeded by OCA on Sat May 3 23:37:30 2008
