2d3n
From Proteopedia
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'''Crystal structure of maltohexaose-producing amylase from Bacillus sp.707 complexed with maltohexaose''' | '''Crystal structure of maltohexaose-producing amylase from Bacillus sp.707 complexed with maltohexaose''' | ||
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[[Category: Kanai, R.]] | [[Category: Kanai, R.]] | ||
[[Category: Yamane, K.]] | [[Category: Yamane, K.]] | ||
| - | [[Category: | + | [[Category: Ligand binding]] |
| - | [[Category: | + | [[Category: Maltohexaose]] |
| - | [[Category: | + | [[Category: Protein-carbohydrate complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:38:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:38, 3 May 2008
Crystal structure of maltohexaose-producing amylase from Bacillus sp.707 complexed with maltohexaose
Overview
Maltohexaose-producing amylase (G6-amylase) from alkalophilic Bacillus sp.707 predominantly produces maltohexaose (G6) in the yield of >30% of the total products from short-chain amylose (DP=17). Our previous crystallographic study showed that G6-amylase has nine subsites, from -6 to +3, and pointed out the importance of the indole moiety of Trp140 in G6 production. G6-amylase has very low levels of hydrolytic activities for oligosaccharides shorter than maltoheptaose. To elucidate the mechanism underlying G6 production, we determined the crystal structures of the G6-amylase complexes with G6 and maltopentaose (G5). In the active site of the G6-amylase/G5 complex, G5 is bound to subsites -6 to -2, while G1 and G6 are found at subsites +2 and -7 to -2, respectively, in the G6-amylase/G6 complex. In both structures, the glucosyl residue located at subsite -6 is stacked to the indole moiety of Trp140 within a distance of 4A. The measurement of the activities of the mutant enzymes when Trp140 was replaced by leucine (W140L) or by tyrosine (W140Y) showed that the G6 production from short-chain amylose by W140L is lower than that by W140Y or wild-type enzyme. The face-to-face short contact between Trp140 and substrate sugars is suggested to regulate the disposition of the glucosyl residue at subsite -6 and to govern product specificity for G6 production.
About this Structure
2D3N is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.
Reference
Role of Trp140 at subsite -6 on the maltohexaose production of maltohexaose-producing amylase from alkalophilic Bacillus sp.707., Kanai R, Haga K, Akiba T, Yamane K, Harata K, Protein Sci. 2006 Mar;15(3):468-77. Epub 2006 Feb 1. PMID:16452622 Page seeded by OCA on Sat May 3 23:38:33 2008
