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| | ==VCP-interacting membrane protein (VIMP)== | | ==VCP-interacting membrane protein (VIMP)== |
| - | <StructureSection load='5kiu' size='340' side='right' caption='[[5kiu]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5kiu' size='340' side='right'caption='[[5kiu]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kiu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KIU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KIU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kiu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KIU FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VIMP, SELS, AD-015, SBBI8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kiu OCA], [http://pdbe.org/5kiu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kiu RCSB], [http://www.ebi.ac.uk/pdbsum/5kiu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kiu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kiu OCA], [https://pdbe.org/5kiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kiu RCSB], [https://www.ebi.ac.uk/pdbsum/5kiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kiu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SELS_HUMAN SELS_HUMAN]] Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.<ref>PMID:15215856</ref> | + | [https://www.uniprot.org/uniprot/SELS_HUMAN SELS_HUMAN] Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.<ref>PMID:15215856</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Tang, W K]] | + | [[Category: Large Structures]] |
| - | [[Category: Xia, D]] | + | [[Category: Tang WK]] |
| - | [[Category: Membrane protein]] | + | [[Category: Xia D]] |
| - | [[Category: P97 adaptor protein]]
| + | |
| - | [[Category: Vcp-interacting membrane protein]]
| + | |
| - | [[Category: Vimp]]
| + | |
| Structural highlights
Function
SELS_HUMAN Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.[1]
Publication Abstract from PubMed
Association of the cytosolic AAA (ATPases associated with various cellular activities) protein p97 to membranes is essential for various cellular processes including endoplasmic reticulum (ER)-associated degradation. The p97 consists of two ATPase domains and an N domain that interacts with numerous cofactors. The N domain of p97 is known to undergo a large nucleotide-dependent conformation switch, but its physiological relevance is unclear. Here we show p97 is recruited to canine ER membranes predominantly by interacting with VCP-interacting membrane protein (VIMP), an ER-resident protein. We found that the recruitment is modulated through a nucleotide-dependent conformation switch of the N domain in wild-type p97, but this modulation is absent in pathogenic mutants. We demonstrate the molecular mechanism of the modulation by a series of structures of p97, VIMP and their complexes and suggest a physiological role of the nucleotide-dependent N domain conformation switch. The lack of modulation in pathogenic mutants is caused by changes in interactions between the N and D1 domain, as demonstrated by multiple intermediate positions adopted by N domains of mutant p97. Our findings suggest the nucleotide-modulated membrane association may also have a role in other p97-dependent processes.
Structural basis for nucleotide-modulated p97 association with the ER membrane.,Tang WK, Zhang T, Ye Y, Xia D Cell Discov. 2017 Dec 12;3:17045. doi: 10.1038/celldisc.2017.45. eCollection, 2017. PMID:29238611[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ye Y, Shibata Y, Yun C, Ron D, Rapoport TA. A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol. Nature. 2004 Jun 24;429(6994):841-7. PMID:15215856 doi:http://dx.doi.org/10.1038/nature02656
- ↑ Tang WK, Zhang T, Ye Y, Xia D. Structural basis for nucleotide-modulated p97 association with the ER membrane. Cell Discov. 2017 Dec 12;3:17045. doi: 10.1038/celldisc.2017.45. eCollection, 2017. PMID:29238611 doi:http://dx.doi.org/10.1038/celldisc.2017.45
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