6f5o
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==A mechanism for the activation of the influenza virus transcriptase== | |
| + | <StructureSection load='6f5o' size='340' side='right' caption='[[6f5o]], [[Resolution|resolution]] 9.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6f5o]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F5O FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5o OCA], [http://pdbe.org/6f5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f5o RCSB], [http://www.ebi.ac.uk/pdbsum/6f5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For transcription, FluPol interacts with the C-terminal domain (CTD) of RNA polymerase II (Pol II), which enables FluPol to snatch capped RNA primers from nascent host RNAs. Here, we describe the co-crystal structure of influenza C virus polymerase (FluPolC) bound to a Ser5-phosphorylated CTD (pS5-CTD) peptide. The position of the CTD-binding site at the interface of PB1, P3, and the flexible PB2 C-terminal domains suggests that CTD binding stabilizes the transcription-competent conformation of FluPol. In agreement, both cap snatching and capped primer-dependent transcription initiation by FluPolC are enhanced in the presence of pS5-CTD. Mutations of amino acids in the CTD-binding site reduce viral mRNA synthesis. We propose a model for the activation of the influenza virus transcriptase through its association with pS5-CTD of Pol II. | ||
| - | + | A Mechanism for the Activation of the Influenza Virus Transcriptase.,Serna Martin I, Hengrung N, Renner M, Sharps J, Martinez-Alonso M, Masiulis S, Grimes JM, Fodor E Mol Cell. 2018 Jun 21;70(6):1101-1110.e4. doi: 10.1016/j.molcel.2018.05.011. Epub, 2018 Jun 14. PMID:29910112<ref>PMID:29910112</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Grimes, J | + | <div class="pdbe-citations 6f5o" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: RNA-directed RNA polymerase]] | ||
| + | [[Category: Grimes, J M]] | ||
| + | [[Category: Martin, I Serna]] | ||
| + | [[Category: Influenza virus rna dependent rna polymerase]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 05:39, 27 June 2018
A mechanism for the activation of the influenza virus transcriptase
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