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5gwn

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Crystal structure of human RCC2

Structural highlights

5gwn is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.309Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RCC2_HUMAN Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.^[1]

Publication Abstract from PubMed

RCC2 (also known as TD60) is a highly conserved protein involved in prognosis in colorectal cancer. However, its relationship with tumor development is less understood. Here we demonstrate a signaling pathway defining regulation of RCC2 and its functions in tumor progression. We report that p53 is a transcriptional regulator of RCC2 that acts through its binding to a palindromic motif in the RCC2 promoter. RCC2 physically interacts and deactivates a small GTPase Rac1 that is known to be involved in metastasis. We solved a high-resolution crystal structure of RCC2 and revealed one RCC1-like domain with a unique beta-hairpin that is requisite for RCC2 interaction with Rac1. p53 or RCC2 deficiency leads to activation of Rac1 and deterioration of extracellular matrix sensing (haptotaxis) of surface-bound gradients. Ectopic expression of RCC2 restores directional migration in p53-null cells. Our results demonstrate that p53 and RCC2 signaling is important for regulation of cell migration and suppression of metastasis. We propose that the p53/RCC2/Rac1 axis is a potential target for cancer therapy.Oncogene advance online publication, 4 September 2017; doi:10.1038/onc.2017.306.

RCC2 is a novel p53 target in suppressing metastasis.,Song C, Liang L, Jin Y, Li Y, Liu Y, Guo L, Wu C, Yun CH, Yin Y Oncogene. 2017 Sep 4. doi: 10.1038/onc.2017.306. PMID:28869598^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mollinari C, Reynaud C, Martineau-Thuillier S, Monier S, Kieffer S, Garin J, Andreassen PR, Boulet A, Goud B, Kleman JP, Margolis RL. The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression. Dev Cell. 2003 Aug;5(2):295-307. PMID:12919680
↑ Song C, Liang L, Jin Y, Li Y, Liu Y, Guo L, Wu C, Yun CH, Yin Y. RCC2 is a novel p53 target in suppressing metastasis. Oncogene. 2017 Sep 4. doi: 10.1038/onc.2017.306. PMID:28869598 doi:http://dx.doi.org/10.1038/onc.2017.306

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5gwn"

Categories: Homo sapiens | Large Structures | Liang L | Yin YX | Yun CH

@@ Line 1: / Line 1: @@
 ==Crystal structure of human RCC2==
-<StructureSection load='5gwn' size='340' side='right' caption='[[5gwn]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
+<StructureSection load='5gwn' size='340' side='right'caption='[[5gwn]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5gwn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5gwn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GWN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.309&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RCC2, KIAA1470, TD60 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwn OCA], [http://pdbe.org/5gwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwn RCSB], [http://www.ebi.ac.uk/pdbsum/5gwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwn OCA], [https://pdbe.org/5gwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gwn RCSB], [https://www.ebi.ac.uk/pdbsum/5gwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RCC2_HUMAN RCC2_HUMAN]] Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.<ref>PMID:12919680</ref>
+[https://www.uniprot.org/uniprot/RCC2_HUMAN RCC2_HUMAN] Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.<ref>PMID:12919680</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Liang, L]]
+[[Category: Large Structures]]
-[[Category: Yin, Y X]]
+[[Category: Liang L]]
-[[Category: Yun, C H]]
+[[Category: Yin YX]]
-[[Category: Protein binding]]
+[[Category: Yun CH]]
-[[Category: Seven-bladed propeller]]

5gwn

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Current revision

Crystal structure of human RCC2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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