2dfc

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[[Image:2dfc.gif|left|200px]]
[[Image:2dfc.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2dfc |SIZE=350|CAPTION= <scene name='initialview01'>2dfc</scene>, resolution 1.19&Aring;
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The line below this paragraph, containing "STRUCTURE_2dfc", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2dfc| PDB=2dfc | SCENE= }}
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|RELATEDENTRY=[[2dfb|2DFB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dfc OCA], [http://www.ebi.ac.uk/pdbsum/2dfc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dfc RCSB]</span>
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}}
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'''Xylanase II from Tricoderma reesei at 293K'''
'''Xylanase II from Tricoderma reesei at 293K'''
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[[Category: Akiba, T.]]
[[Category: Akiba, T.]]
[[Category: Harata, K.]]
[[Category: Harata, K.]]
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[[Category: bent beta sheet]]
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[[Category: Bent beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:18:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:33:58 2008''
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Revision as of 21:18, 3 May 2008

Image:2dfc.gif

Template:STRUCTURE 2dfc

Xylanase II from Tricoderma reesei at 293K


Overview

An orthorhombic crystal of xylanase II from Trichoderma reesei was grown in the presence of sodium iodide. Crystal structures at atomic resolution were determined at 100 and 293 K. Protein molecules were aligned along a crystallographic twofold screw axis, forming a helically extended polymer-like chain mediated by an iodide ion. The iodide ion connected main-chain peptide groups between two adjacent molecules by an N-H...I-...H-N hydrogen-bond bridge, thus contributing to regulation of the molecular arrangement and suppression of the rigid-body motion in the crystal with high diffraction quality. The structure at 293 K showed considerable thermal motion in the loop regions connecting the beta-strands that form the active-site cleft. TLS model analysis of the thermal motion and a comparison between this structure and that at 100 K suggest that the fluctuation of these loop regions is attributable to the hinge-like movement of the beta-strands.

About this Structure

2DFC is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement., Watanabe N, Akiba T, Kanai R, Harata K, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):784-92. Epub 2006, Jun 20. PMID:16790934 Page seeded by OCA on Sun May 4 00:18:56 2008

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