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5xrm

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Revision as of 06:18, 8 March 2018

Galectin-10/Charcot-Leyden crystal protein variant W72A crystal structure

Structural highlights

5xrm is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	CLC, LGALS10, LGALS10A (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LEG10_HUMAN] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).^[1]

Publication Abstract from PubMed

Galectin-10 (Gal-10) which forms Charcot-Leyden crystals in vivo, is crucial to regulating lymph cell function. Here, we solved the crystal structures of Gal-10 and eight variants at resolutions of 1.55-2.00 A. Structural analysis and size exclusion chromatography demonstrated that Gal-10 dimerizes with a novel global shape that is different from that of other prototype galectins (e.g., Gal-1, -2 and -7). In the Gal-10 dimer, Glu33 from one subunit modifies the carbohydrate binding site of another, essentially inhibiting disaccharide binding. Nevertheless, glycerol (and possibly other small hydroxylated molecules) can interact with residues at the ligand binding site, with His53 being the most crucial for binding. Alanine substitution of the conserved Trp residue (Trp72) that is crucial to saccharide binding in other galectins, actually leads to enhanced erythrocyte agglutination, suggesting that Trp72 negatively regulates Gal-10 ligand binding. Overall, our crystallographic and biochemical results provide insight into Gal-10 ligand binding specificity.

Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding.,Su J, Gao J, Si Y, Cui L, Song C, Wang Y, Wu R, Tai G, Zhou Y Glycobiology. 2017 Dec 23. pii: 4773962. doi: 10.1093/glycob/cwx107. PMID:29293962^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H. Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function. Blood. 2007 Sep 1;110(5):1550-8. doi: 10.1182/blood-2007-01-069229. Epub 2007 May, 14. PMID:17502455 doi:http://dx.doi.org/10.1182/blood-2007-01-069229
↑ Su J, Gao J, Si Y, Cui L, Song C, Wang Y, Wu R, Tai G, Zhou Y. Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding. Glycobiology. 2017 Dec 23. pii: 4773962. doi: 10.1093/glycob/cwx107. PMID:29293962 doi:http://dx.doi.org/10.1093/glycob/cwx107

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xrm"

Categories: Human | Su, J | Galectin-10/charcot-leyden crystal protein | Protein binding

@@ Line 3: / Line 3: @@
 <StructureSection load='5xrm' size='340' side='right' caption='[[5xrm]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xrm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xrm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRM FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLC, LGALS10, LGALS10A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrm OCA], [http://pdbe.org/5xrm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrm RCSB], [http://www.ebi.ac.uk/pdbsum/5xrm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrm ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Su, J]]
 [[Category: Galectin-10/charcot-leyden crystal protein]]
 [[Category: Protein binding]]

5xrm

From Proteopedia

Revision as of 06:18, 8 March 2018

Galectin-10/Charcot-Leyden crystal protein variant W72A crystal structure

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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