2dog
From Proteopedia
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'''Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8''' | '''Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8''' | ||
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[[Category: Tatsuguchi, A.]] | [[Category: Tatsuguchi, A.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: National project on protein structural and functional analyse]] |
| - | [[Category: | + | [[Category: Nppsfa]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Unknown function]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:52:46 2008'' | |
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Revision as of 21:52, 3 May 2008
Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8
Overview
The RimM protein has been implicated in the maturation of the 30S ribosomal subunit. It binds to ribosomal protein S19, located in the head domain of the 30S subunit. Multiple sequence alignments predicted that RimM possesses two domains in its N- and C-terminal regions. In the present study, we have produced Thermus thermophilus RimM in both the full-length form (162 residues) and its N-terminal fragment, spanning residues 1 to 85, as soluble proteins in Escherichia coli and have performed structural analyses by nuclear magnetic resonance spectroscopy. Residues 1 to 80 of the RimM protein fold into a single structural domain adopting a six-stranded beta-barrel fold. On the other hand, the C-terminal region of RimM (residues 81 to 162) is partly folded in solution. Analyses of 1H-15N heteronuclear single quantum correlation spectra revealed that a wide range of residues in the C-terminal region, as well as the residues in the vicinity of a hydrophobic patch in the N-terminal domain, were dramatically affected upon complex formation with ribosomal protein S19.
About this Structure
2DOG is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structural characterization of the ribosome maturation protein, RimM., Suzuki S, Tatsuguchi A, Matsumoto E, Kawazoe M, Kaminishi T, Shirouzu M, Muto Y, Takemoto C, Yokoyama S, J Bacteriol. 2007 Sep;189(17):6397-406. Epub 2007 Jul 6. PMID:17616598 Page seeded by OCA on Sun May 4 00:52:46 2008
Categories: Single protein | Thermus thermophilus | Kaminishi, T. | Kawazoe, M. | Matsumoto, E. | Muto, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shirouzu, M. | Suzuki, S. | Takemoto, C. | Tatsuguchi, A. | Yokoyama, S. | Beta barrel | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Unknown function
